Oncotarget

Research Papers:

Long non-coding RNA PTENP1 functions as a ceRNA to modulate PTEN level by decoying miR-106b and miR-93 in gastric cancer

Rupeng Zhang _, Yuenan Guo, Zhenchi Ma, Gang Ma, Qiang Xue, Fangxuan Li and Liren Liu

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Oncotarget. 2017; 8:26079-26089. https://doi.org/10.18632/oncotarget.15317

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Abstract

Rupeng Zhang1,4,5, Yuenan Guo1,4,5, Zhenchi Ma1,4,5, Gang Ma2,4,5, Qiang Xue1,4,5, Fangxuan Li3,4,5, Liren Liu2,4,5

1Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China

2Department of Gastrointestinal Cancer Biology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China

3Department of Cancer Prevention Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China

4National Clinical Research Center of Cancer, Tianjin 300060, China

5Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China

Correspondence to:

Rupeng Zhang, email: tijzhangrp@163.com

Liren Liu, email: liuliren@tjmuch.com

Keywords: PTENP1, miR-106b, miR-93, long non-coding RNA, gastric cancer

Received: October 20, 2016     Accepted: January 29, 2017     Published: February 13, 2017

ABSTRACT

Recent studies have shown that competing endogenous RNAs (ceRNAs) play an important role in the regulation of gene expression, and participate in a wide range of biological processes, including carcinogenesis. Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, has been reported to exert its tumor suppressive function via modulation of PTEN expression in many malignancies. However, whether a PTENP1~miRNA~PTEN ceRNA network exists and how it functions in gastric cancer (GC) remains elusive. In order to identify and characterize the PTENP1~miRNA~PTEN ceRNA network in GC, we first determined PTENP1 levels in clinical GC samples and found that PTENP1 and PTEN were concurrently downregulated in these samples. We further demonstrated that PTENP1 could act as a ceRNA to sponge miR-106b and miR-93 from targeting PTEN for downregulation using a novel ceRNA in vitro gradient assay. Thus, we revealed a tumor suppressive role of PTENP1 as ceRNA in GC and pinpointed the specific miRNAs decoyed by PTENP1, highlighting the emerging roles of ceRNAs in the biological regulation of GC cells and their possible clinical significance.


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