Generation of in vivo neural stem cells using partially reprogrammed cells defective in in vitro differentiation potential
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Jong Soo Kim1,*, Yean Ju Hong1,*, Hyun Woo Choi1, Hyuk Song1, Sung June Byun2, Jeong Tae Do1
1Department of Stem Cell and Regenerative Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea
2Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea
*These authors contributed equally to this work
Jeong Tae Do, email: email@example.com
Keywords: teratoma, pluripotent stem cells, iPSCs, neural stem cells, differentiation
Received: September 08, 2016 Accepted: January 16, 2017 Published: January 27, 2017
Pluripotent stem cells can be easily differentiated in vitro into a certain lineage through embryoid body formation. Recently, however, we reported partially reprogrammed cells showing some pluripotent characteristics, which failed to differentiate in vitro. Here, we attempted to generate neural stem cells (NSCs) from partially reprogrammed cells using an in vivo differentiation system involving teratoma formation. Partially reprogrammed cells formed teratomas after injection into immunocompromised mice, and NSCs could be isolated from these teratomas. These in vivo NSCs expressed NSC markers and terminally differentiated into neurons and glial cells. Moreover, these NSCs exhibited molecular profiles very similar to those of brain-derived NSCs. These results suggest that partially reprogrammed cells defective in in vitro differentiation ability can differentiate into pure populations of NSCs through an in vivo system.
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