Research Papers:
High expression of miR-105-1 positively correlates with clinical prognosis of hepatocellular carcinoma by targeting oncogene NCOA1
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Abstract
Yu-Shui Ma1,2,*, Ting-Miao Wu1,3,*, Zhong-Wei Lv1,*, Gai-Xia Lu1,*, Xian-Ling Cong4,*, Ru-Ting Xie5, Hui-Qiong Yang5, Zheng-Yan Chang5, Ran Sun4, Li Chai1, Ming-Xiang Cai1, Xiao-Jun Zhong6, Jian Zhu7, Da Fu8
1Department of Nuclear Medicine, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
2Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China
3Department of Radiology, The Fourth Affiliated Hospital, Medical University of Anhui, Hefei 230601, China
4Tissue Bank, China-Japan Union Hospital, Jilin University, Changchun 130033, China
5Department of Pathology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
6Department of Medical Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China
7Department of Digestive Surgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
8Central Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
*These authors have contributed equally to this work
Correspondence to:
Xiao-Jun Zhong, email: [email protected]
Jian Zhu, email: [email protected]
Da Fu, email: [email protected]
Keywords: miR-105-1, HCC, biomarker, survival, target
Received: September 22, 2016 Accepted: December 21, 2016 Published: January 02, 2017
ABSTRACT
Increasing evidence supports that microRNA (miRNA) plays a significant functional role in cancer progression by directly regulating respective targets. In this study, the expression levels of miR-105-1 and its target gene were analyzed using genes microarray and hierarchical clustering analysis followed by validation with quantitative RT-PCR in hepatocellular carcinoma (HCC) and normal liver tissues. We examined the expression of nuclear receptor coactivator 1 (NCOA1), the potential target gene of miR-105-1, following the transfection of miR-105-1 mimics or inhibitors. Our results showed that miR-105-1 was downregulated in HCC tissues when compared with normal liver tissues and patients with lower miR-105-1 expression had shorter overall survival (OS) and progression free survival (PFS). Moreover, NCOA1 was confirmed to be a direct target of miR-105-1. Furthermore, concomitant high expression of NCOA1 and low expression of miR-105-1 correlated with a shorter median OS and PFS in HCC patients. In conclusion, our results provide the first evidence that NCOA1 is a direct target of miR-105-1 suggesting that NCOA1 and miR-105-1 may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of HCC patients.
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