Oncotarget

Research Papers: Gerotarget (Focus on Aging):

SS31 Ameliorates age-related activation of NF-κB signaling in senile mice model, SAMP8

Zhi-Hua Hao, Yue Huang, Mei-Rong Wang, Tian-Tian Huo, Qian Jia, Rong-Fang Feng, Ping Fan and Jian-Hua Wang _

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Oncotarget. 2017; 8:1983-1992. https://doi.org/10.18632/oncotarget.14077

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Abstract

Zhi-Hua Hao1,2, Yue Huang3, Mei-Rong Wang1,2, Tian-Tian Huo1, Qian Jia2, Rong-Fang Feng1, Ping Fan2 and Jian-Hua Wang1

1 Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei, China

2 Graduate School,Hebei Medical University, Shijiazhuang, Hebei, China

3 School of Medical Sciences, Faculty of Medicine, UNSW Australia, Sydney, Australia

Correspondence to:

Jian-Hua Wang, email:

Keywords: aging, SAMP8, inflammation, SS31, NF-κB, Gerotarget

Received: March 31, 2016 Accepted: December 12, 2016 Published: December 21, 2016

Abstract

Aging has been attributed to oxidative stress and inflammatory response, in which NF-κB and Nrf2-ARE signaling pathways play significant roles. Senescence accelerated mouse prone 8 (SAMP8) is generally used an animal model for aging studies. Here, we investigated the NF-κB and Nrf2-ARE signaling pathways in SAMP8 brains at different ages and their responses to SS31 peptide treatment. Thirty six SAMP8 mice were separated into aging groups and SS31-treatment groups. The hippocampus from each mouse was dissected for RNA and protein extraction. Cytokines and ROS levels were measured using ELISA and standardised method. Gene expressions of NF-κB, Nrf2 and HO-1 were measured by RT-qPCR. Total protein amount of NF-κB and HO-1, as well as the concentrations of nuclear and cytoplasmic Nrf2 were measured using Western blots. Our data showed that aging could activate both NF-κB and Nrf2-ARE signaling pathways, which could be suppressed and activated by SS31 treatment respectively. Regression analysis revealed that NF-κB gene expression was the most important parameter predicting aging process and SS31 treatment effects in SAMP8. Our findings suggested that SS31 treatment may modulate the inflammatory and oxidative stress status of the aged brains and exert protective effects during brain aging.


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