Oncotarget

Research Papers: Immunology:

Dysbiosis of urinary microbiota is positively correlated with Type 2 diabetes mellitus

Fengping Liu, Zongxin Ling, Yonghong Xiao, Longxian Lv, Qing Yang, Baohong Wang, Haifeng Lu, Li Zheng, Ping Jiang, Wei Wang _ and Lanjuan Li

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Oncotarget. 2017; 8:3798-3810. https://doi.org/10.18632/oncotarget.14028

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Abstract

Fengping Liu1,4,*, Zongxin Ling2,3,*, Yonghong Xiao2,3,*, Longxian Lv2,3, Qing Yang3, Baohong Wang2,3, Haifeng Lu2,3, Li Zheng1, Ping Jiang1, Wei Wang1,** and Lanjuan Li2,3,**

1 Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

2 State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, China

3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, China

4 Yancheng Medical College, Yancheng, Jiangsu, China

* These authors have contributed equally to this work

** These authors also have contributed equally to this work

Correspondence to:

Wei Wang, email:

Lanjuan Li, email:

Keywords: Akkermansia muciniphila; Escherichia coli; lactobacillus; type 2 diabetes mellitus; urinary microbiota; Immunology and Microbiology Section, Immune response, Immunity

Received: November 02, 2016 Accepted: December 13, 2016 Published: December 19, 2016

Abstract

Type 2 diabetes mellitus (T2DM) may be associated with altered urinary microbiota in female patients. We investigated alterations of urinary microbiota in Chinese female T2DM patients, and explored the associations between urinary microbiota and a patient’s fasting blood glucose (FBG), urine glucose (UGLU), age, menstrual status, and body mass index (BMI). Midstream urine was collected from 70 female T2DM patients and 70 healthy females. Microbial diversity and composition were analyzed using the Illumina MiSeq sequencing platform by targeting the hypervariable V3-V4 regions of the 16S rRNA gene. We found that bacterial diversity was decreased in T2DM patients. Increased Actinobacteria phylum was positively correlated with FBG, UGLU, and BMI; Lactobacillus abundance decreased with age and menopause; and increased Lactobacillus correlated positively with FBG and UGLU. Decreased Akkermansia muciniphila was associated with FBG and UGLU. Escherichia coli abundance did not differ between the two cohorts. Carbohydrate and amino acid metabolism was reduced in T2DM patients, which were associated with bacterial richness indices such as Chao1 and ACE. Detailed microbiota analysis of well-characterized T2DM patients and healthy controls indicate that Chinese T2DM female patients exhibit dysbiosis of urinary microbiota.


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