ED-B fibronectin expression is a marker of epithelial-mesenchymal transition in translational oncology
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Iacopo Petrini1, Serena Barachini2, Vittoria Carnicelli3, Sara Galimberti2, Letizia Modeo4, Roberto Boni4, Martina Sollini5, Paola Anna Erba4
1General Pathology, Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy
2Laboratory of Hematology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
3Biochemistry, Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy
4Nuclear Medicine, Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy
5Department of Biomedical Sciences, Humanitas University, Rozzano, Milan, Italy
Iacopo Petrini, email: firstname.lastname@example.org
Keywords: fibronectin, endothelial-to-mesenchymal transition, ED-B, prostate cancer, TGF-β
Received: July 25, 2016 Accepted: November 09, 2016 Published: November 25, 2016
Fibronectin is a component of the extracellular matrix that links collagen fibers to integrins on the cell’s surface. The splicing isoforms, containing the ED-B domain, are not expressed in adult tissues but only in tumor stroma or during embryonic development. Fibroblasts and endothelial cells express ED-B fibronectin during angiogenesis. Also cancer cells can synthetize ED-B fibronectin, but its function in tumor growth needs to be further elucidated.
We evaluated the expression of ED-B fibronectin in prostate cancer cell lines: PC3 and DU145. Using TGF-β, we induced epithelial to mesenchymal transition in culture and observed an increase of ED-B fibronectin expression. Thereafter, we evaluated the expression of ED-B fibronectin in multipotent mesangiogenic progenitor cells, and in mesenchymal stromal cells. The expression of ED-B fibronectin was much higher in mesenchymal than prostate cancer cells even after the epithelial to mesenchymal transition.
Epithelial to mesenchymal transition is a key step for tumor progression contributing to the metastatic spread. Therefore, circulating cancer cells could seed into the metastatic niche taking advantage from the ED-B fibronectin that secrete their own.
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