Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study
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Xia Lingzi1,2, Yin Zhihua1,2, Li Xuelian1,2, Ren Yangwu1,2, Zhang Haibo3, Zhao Yuxia4, Zhou Baosen1,2
1Department of Epidemiology, China Medical University, Shenyang, Liaoning, 110122, Peoples R China
2Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Province Department of Education, Shenyang, Liaoning, 110122, Peoples R China
3Department of Radiotherapy, Shenyang North Hospital, Shenyang, Liaoning, 110001, Peoples R China
4Department of Radiotherapy Oncology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, Peoples R China
Zhou Baosen, email: email@example.com
Keywords: miRNA, SNP, lung cancer, prognosis, Chinese
Received: March 22, 2016 Accepted: October 17, 2016 Published: November 04, 2016
To investigate the prognostic effect of microRNA single nucleotide polymorphisms (SNP) on non-small cell lung cancer (NSCLC) patients, 658 female participants from northeast China were enrolled in our prospective cohort study and followed up from 2010 to 2015. C-containing genotypes of miR-149 rs2292832 were associated with better overall survival (OS). The joint effect of miR-149 and miR-196a2 and the joint effect of miR-149 and miR-608 were also observed in our study. To verify the function of miR-149 rs2292832, A549 cell lines were stably transfected with lenti-virus containing miR-149-C vector, miR-149-T vector and empty vector. Cells containing C allele assumed a higher expression level of miR-149, a decrease in cell growth and the sensitivity to anticancer drug when compared with cells containing T allele. The role of miR-149 playing in cancer prognosis may function through DNA topoisomerases 1 (TOP1) pathway, according to the results from luciferase reporter assays. In conclusion, miR-149 C allele may be a prognostic biomarker for better NSCLC OS.
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