Research Papers:

Nickel chloride-induced apoptosis via mitochondria- and Fas-mediated caspase-dependent pathways in broiler chickens

Hongrui Guo, Hengmin Cui _, Jing Fang, Zhicai Zuo, Junliang Deng, Xun Wang, Ling Zhao, Bangyuan Wu, Kejie Chen and Jie Deng

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Oncotarget. 2016; 7:79747-79760. https://doi.org/10.18632/oncotarget.12946

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Hongrui Guo1, Hengmin Cui1,2, Jing Fang1,2, Zhicai Zuo1,2, Junliang Deng1,2, Xun Wang1,2, Ling Zhao1,2, Bangyuan Wu1, Kejie Chen1, Jie Deng1

1College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China

2Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China

Correspondence to:

Hengmin Cui, email: cui580420@sicau.edu.cn

Keywords: NiCl2, apoptosis, mitochondria-mediated caspase-dependent apoptosis, Fas-mediated caspase-dependent apoptosis, kidney

Received: July 02, 2016    Accepted: October 13, 2016    Published: October 27, 2016


Ni, a metal with industrial and commercial uses, poses a serious hazard to human and animal health. In the present study, we used flow cytometry, immunohistochemistry and qRT-PCR to investigate the mechanisms of NiCl2-induced apoptosis in kidney cells. After treating 280 broiler chickens with 0, 300, 600 or 900 mg/kg NiCl2 for 42 days, we found that two caspase-dependent pathways were involved in the induced renal tubular cell apoptosis. In the mitochondria-mediated caspase-dependent apoptotic pathway, cyt-c, HtrA2/Omi, Smac/Diablo, apaf-1, PARP, and caspase-9, 3, 6 and 7 were all increased, while. XIAP transcription was decreased. Concurrently, in the Fas-mediated caspase-dependent apoptotic pathway, Fas, FasL, caspase-8, caspase-10 and Bid levels were all increased. These results indicate that dietary NiCl2 at 300+ mg/kg induces renal tubular cell apoptosis in broiler chickens, involving both mitochondrial and Fas-mediated caspase-dependent apoptotic pathways. Our results provide novel insight into Ni and Ni-compound toxicology evaluated in vitro and in vivo.

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