Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Ampelopsin attenuates brain aging of D-gal-induced rats through miR-34a-mediated SIRT1/mTOR signal pathway

Xianjuan Kou, Xingran Liu, Xianbing Chen, Jie Li, Xiaoqi Yang, Jingjing Fan, Yi Yang and Ning Chen _

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Oncotarget. 2016; 7:74484-74495. https://doi.org/10.18632/oncotarget.12811

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Abstract

Xianjuan Kou1,*, Xingran Liu2,*, Xianbing Chen3, Jie Li2, Xiaoqi Yang2, Jingjing Fan1, Yi Yang1 and Ning Chen1

1 Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, China

2 Graduate School, Wuhan Sports University, Wuhan, China

3 College of Medicine, Hubei University for Nationalities, Enshi, China

* These authors have contributed equally to this work

Correspondence to:

Ning Chen, email:

Keywords: ampelopsin, aging, autophagy, miR-34a, SIRT1-mTOR signal pathway, Gerotarget

Received: July 15, 2016 Accepted: October 17, 2016 Published: October 21, 2016

Abstract

The underlying molecular mechanisms for aging-related neurodegenerative diseases such as Alzheimer’s disease (AD) are not fully understood. Currently, growing evidences have revealed that microRNAs (miRNAs) are involved in aging and aging-related diseases. The up-regulation of miR-34a has been reported to be associated with aging-related diseases, and thus it should be a promising therapeutic target. Ampelopsin, also called dihydromyricetin (DHM), a natural flavonoid from Chinese herb Ampelopsis grossedentata, has been reported to possess multiple pharmacological functions including anti-inflammatory, anti-oxidative and anti-cancer functions. Meanwhile, it has also gained tremendous attention against neurodegenerative diseases as an anti-aging compound. In the present study, the model rats with D-gal-induced brain aging revealed an obvious expression of miR-34a; in contrast, it could be significantly suppressed upon DHM treatment. In addition, target genes associated with miR-34a in the presence of DHM treatment were also explored. DHM supplementation inhibited D-gal-induced apoptosis and rescued impaired autophagy of neurons in hippocampus tissue. Moreover, DHM activated autophagy through up-regulated SIRT1 and down-regulated mTOR signal pathways due to the down-regulated miR-34a. In conclusion, DHM can execute the prevention and treatment of D-gal-induced brain aging by miR-34a-mediated SIRT1-mTOR signal pathway.


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