14-3-3ζ/TGFβR1 promotes tumor metastasis in lung squamous cell carcinoma
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Yanbin Zhao1,*, Wenbo Qiao2,*, Xiaoyuan Wang1,*, Hang Yin2, Jianqi Cui1, Yue Cui1, Xuesong Chen1, Jing Hu1, Hailing Lu1, Qingwei Meng1, Yan Wang1, Li Cai1
1The Department of Internal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang Province, China
2The Department of radiotherapy, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang Province, China
*These three authors contributed equally to this work
Li Cai, email: email@example.com
Yan Wang, email: firstname.lastname@example.org
Keywords: 14-3-3ζ, TGFβR1, metastasis, lung squamous cell carcinoma, EMT
Received: June 06, 2016 Accepted: September 17, 2016 Published: October 15, 2016
14-3-3ζ is involved in tumor cell growth and apoptosis. However, the mechanism of 14-3-3ζ in lung squamous cell carcinoma (SCC) metastasis has not been illuminated. In our studies, we found that the expression of 14-3-3ζ was highly expressed in lung SCC compared to normal lung tissues. High expression of 14-3-3ζ was associated with pTNM stage (p<0.05) and lymph node metastasis (p<0.05). Furthermore, the expression of 14-3-3ζ protein was associated with high levels of TGFβR1 protein (p=0.005), and pSMAD3 (p=0.033). Lung SCC patients with high 14-3-3ζ expression have significantly shorter OS and DFS compared to patients with low 14-3-3ζ expression. Additionally, 14-3-3ζ knockdown inhibited cell proliferation, migratory and invasive properties of human lung SCC cells. TGFβR1 was involved in 14-3-3ζ-mediated cell proliferation and metastasis of lung SCC cells. Additionally, sh-14-3-3ζ can suppress tumor growth and metastasis in vivo. Thus, these data provide the evidence that 14-3-3ζ promote tumor metastasis and might be a prognostic biomarker and target for therapeutic strategy in lung SCC.
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