The first investigation of Wilms’ tumour atomic structure-nitrogen and carbon isotopic composition as a novel biomarker for the most individual approach in cancer disease
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Katarzyna Taran1, Tomasz Frączek2, Anita Sikora-Szubert3, Anna Sitkiewicz4, Wojciech Młynarski5, Józef Kobos6, Piotr Paneth2
1Department of Pathology, Medical University of Lodz, Poland
2Institute of Applied Radiation Chemistry, Lodz University of Technology, Poland
3Clinic of High Risk Pregnancy, Medical University of Lodz, Poland
4Department of Oncology and Paediatric Surgery, Konopnicka Memorial Hospital, Medical University of Lodz, Poland
5Department of Pediatrics, Oncology, Hematology and Diabetology, Konopnicka Memorial Hospital, Medical University of Lodz, Poland
6Department of Paediatric Pathology, Medical University of Lodz, Poland
Katarzyna Taran, email: firstname.lastname@example.org
Keywords: Wilms’ tumour, stable isotopes, prognosis, anaplasia, personalized medicine
Received: June 09, 2016 Accepted: October 03, 2016 Published: October 08, 2016
The paper describes a novel approach to investigating Wilms’ tumour (nephroblastoma) biology at the atomic level. Isotope Ratio Mass Spectrometry (IRMS) was used to directly assess the isotope ratios of nitrogen and carbon in 84 Wilms’ tumour tissue samples from 28 cases representing the histological spectrum of nephroblastoma. Marked differences in nitrogen and carbon isotope ratios were found between nephroblastoma histological types and along the course of cancer disease, with a breakout in isotope ratio of the examined elements in tumour tissue found between stages 2 and 3. Different isotopic compositions with regard to nitrogen and carbon content were observed in blastemal Wilms’ tumour, with and without focal anaplasia, and in poorly- and well-differentiated epithelial nephroblastoma. This first assessment of nitrogen and carbon isotope ratio reveals the previously unknown part of Wilms’ tumour biology and represents a potential novel biomarker, allowing for a highly individual approach to treating cancer. Furthermore, this method of estimating isotopic composition appears to be the most sensitive tool yet for cancer tissue evaluation, and a valuable complement to established cancer study methods with prospective clinical impact.
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