Chicken  GHR  natural antisense transcript regulates  GHR  mRNA in LMH cells

Li Zhang; Shudai Lin; Lilong An; Jinge Ma; Fengfang Qiu; Rumin Jia; Qinghua Nie; Dexiang Zhang; Qingbin Luo; Ting Li; Zhang Wang; Xiquan Zhang

doi:10.18632/oncotarget.12437

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Research Papers:

Chicken GHR natural antisense transcript regulates GHR mRNA in LMH cells

Li Zhang, Shudai Lin, Lilong An, Jinge Ma, Fengfang Qiu, Rumin Jia, Qinghua Nie, Dexiang Zhang, Qingbin Luo, Ting Li, Zhang Wang and Xiquan Zhang _

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Oncotarget. 2016; 7:73607-73617. https://doi.org/10.18632/oncotarget.12437

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Abstract

Li Zhang1,2,*, Shudai Lin1,*, Lilong An2, Jinge Ma1, Fengfang Qiu1, Rumin Jia2, Qinghua Nie1, Dexiang Zhang1, Qingbin Luo1, Ting Li2, Zhang Wang2, Xiquan Zhang1

1Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science of South China Agricultural University, Guangzhou 510642, P.R. China

2Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R. China

*These authors contributed equally to this work

Correspondence to:

Xiquan Zhang, email: [email protected]

Keywords: chicken, GHR natural antisense transcript, GHR mRNA, liver, LMH cell

Received: June 07, 2016 Accepted: September 21, 2016 Published: October 04, 2016

ABSTRACT

Growth hormone receptor (GHR) played key roles in human and animal growth. Both human laron type dwarfism and sex linked dwarf chicken were caused by the mutation of GHR gene. In this study, we identified an endogenously expressed long non-coding natural antisense transcript, GHR-AS, which overlapped with the GHR mRNA (GHR-S) in a tail to tail manner. Spatial and temporal expression analyses indicated that GHR-AS were highly expressed in chicken liver and displayed ascending with the development of chicken from E10 to 3 w of age. Interfering GHR-AS caused GHR-S decreasing, accompanied with increasing of the inactive gene indicator, H3K9me2, in the GHR-S promoter regions in LMH cells. RNase A experiment exhibited that GHR-AS and GHR-S can form double strand RNAs at the last exon of GHR gene in vivo and in vitro, which hinted they could act on each other via the region. In addition, the levels of GHR-S and GHR-AS can be affected by DNA methylation. Compared the normal chicken with the dwarfs, the negative correlation trends were showed between the GHR-S promoter methylation status and the GHR-AS levels. This is the first report of that GHR gene possessed natural antisense transcript and the results presented here further highlight the fine and complicated regulating mechanism of GHR gene in chicken development.

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Research Papers:

Chicken GHR natural antisense transcript regulates GHR mRNA in LMH cells

Abstract