Oncotarget

Research Papers: Pathology:

Adipocyte miR-200b/a/429 ablation in mice leads to high-fat-diet-induced obesity

Cong Tao, Hongyan Ren, Pan Xu, Jia Cheng, Sujuan Huang, Rong Zhou, Yulian Mu, Shulin Yang, Desheng Qi, Yanfang Wang, Kui Li _

PDF |  HTML  |  Supplementary Files  |  Order a Reprint

Oncotarget. 2016; 7:67796-67807. https://doi.org/10.18632/oncotarget.12080

Metrics: PDF 702 views  |   HTML 560 views  |   ?  


Abstract

Cong Tao1,2,*, Hongyan Ren1,3,*, Pan Xu1, Jia Cheng1, Sujuan Huang1, Rong Zhou1, Yulian Mu1, Shulin Yang1, Desheng Qi2, Yanfang Wang1 and Kui Li1

1 State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

2 College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China

3 Key Laboratory of Animal Embryo & Molecular Breeding of Hubei Province, Institute of Veterinary and Animal Science, Hubei Academy of Agricultural Science, Wuhan, China

* These authors have contributed equally to this work

Correspondence to:

Kui Li, email:

Yanfang Wang, email:

Keywords: miR-200b/a/429, insulin resistance, high-fat-diet, knockout, adipose tissue, Pathology Section

Received: March 20, 2016 Accepted: September 12, 2016 Published: September 16, 2016

Abstract

Growing evidence demonstrates the important role of microRNAs (miRs) in regulating adipogenesis, obesity and insulin resistance. The miR-200b/a/429 cluster has been functionally characterized in mammalian reproduction; however, the potential role of the miR-200 family in adipocytes is poorly understood. The aim of our study was to investigate the physiological function of miR-200b/a/429 in the regulation of whole-body metabolism in terms of the activities and targets of this cluster in adipocytes. We generated adipocyte-specific miR-200b/a/429 knockout (ASKO) mice using a Cre-loxP system in which Cre expression was driven by the aP2 promoter. The ASKO and wild type (WT) littermate were fed a chow diet (CD) or high-fat-diet (HFD), and changes in body composition, metabolic parameters, energy homeostasis, glucose tolerance and insulin sensitivity were analyzed. The miR-200b/a/429 putative target genes were predicted and validated via luciferase reporter assays. We found that the HFD-fed ASKO mice gradually gained more body weight than the WT mice due to the increased adiposity. Decreased glucose tolerance and insulin sensitivity were also observed in the HFD-fed ASKO mice. Notably, the down-regulation of lipolysis-related genes and the decreased response to CL-316,243 stimulation in the HFD-fed ASKO mice suggested that these animals exhibited impaired lipolysis. In addition, the HFD-fed ASKO mice displayed impaired energy expenditure, indicating that the miR-200b/a/429 cluster is essential for developing adaptive responses to stressors such as HFD. For the first time, our studies demonstrated the essential role of miR-200b/a/429 in adipocytes in the regulation of HFD-induced whole-body metabolic changes.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 12080