GADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens
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Xinheng Zhang1,2, Zhuanqiang Yan1, Xinjian Li1,2, Wencheng Lin1,2,3, Zhenkai Dai1,2, Yiming Yan1,2, Piaopiao Lu1,2, Weiguo Chen1,2,3, Huanmin Zhang4, Feng Chen1,2,3, Jingyun Ma1,2, Qingmei Xie1,2,3
1College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China
2Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China
3South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China
4USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, U.S.A
Qingmei Xie, email: email@example.com
Keywords: ALV-J, RNA-Seq, differentially expressed genes, GADD45β, viral replication
Received: June 20, 2016 Accepted: September 05, 2016 Published: September 15, 2016
Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45β inhibited ALV-J replication. GADD45β could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.
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