Oncotarget

Research Papers:

Interferon-stimulated gene of 20 kDa protein (ISG20) degrades RNA of hepatitis B virus to impede the replication of HBV in vitro and in vivo

Chean Ring Leong, Kenji Funami, Hiroyuki Oshiumi, Deng Mengao, Hiromi Takaki, Misako Matsumoto, Hussein H. Aly, Koichi Watashi, Kazuaki Chayama, Tsukasa Seya _

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Oncotarget. 2016; 7:68179-68193. https://doi.org/10.18632/oncotarget.11907

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Abstract

Chean Ring Leong1,4,*, Kenji Funami1,*, Hiroyuki Oshiumi1,5, Deng Mengao1, Hiromi Takaki1, Misako Matsumoto1, Hussein H. Aly2, Koichi Watashi2, Kazuaki Chayama3, Tsukasa Seya1

1Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638 Japan

2Department of Virology II, National Institute of Infectious Diseases, Tokyo, 162-8640 Japan

3Department of Gastroenterology and Metabolism, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, 734-8553 Japan

4Present address: Section of Bioengineering Technology, Universiti Kuala Lumpur (UNIKL) MICET, Kuala Lumpur, 78000 Malaysia

5Present address: Department of Immunology, Graduate School of Medical Science, Kumamoto University, Kumamoto, 860-8556 Japan

*These authors have contributed equally to this work

Correspondence to:

Tsukasa Seya, email: seya-tu@pop.med.hokudai.ac.jp

Keywords: hepatitis B virus (HBV), interferon (IFN), interferon-stimulated genes (ISGs), ISG20, gene therapy

Received: June 15, 2016     Accepted: August 15, 2016     Published: September 08, 2016

ABSTRACT

Hepatitis B virus (HBV) barely induces host interferon (IFN)-stimulated genes (ISGs), which allows efficient HBV replication in the immortalized mouse hepatocytes as per human hepatocytes. Here we found that transfection of Isg20 plasmid robustly inhibits the HBV replication in HBV-infected hepatocytes irrespective of IRF3 or IFN promoter activation. Transfection of Isg20 is thus effective to eradicate HBV in the infected hepatocytes. Transfection of HBV genome or ε-stem of HBV pgRNA (active pgRNA moiety) failed to induce Isg20 in the hepatocytes, while control polyI:C (a viral dsRNA analogue mimic) activated MAVS pathway leading to production of type I IFN and then ISGsg20 via the IFN-α/β receptor (IFNAR). Consistently, addition of IFN-α induced Isg20 and partially suppressed HBV replication in hepatocytes. Chasing HBV RNA, DNA and proteins by blotting indicated that ISG20 expression decreased HBV RNA and replicative DNA in HBV-transfected cells, which resulted in low HBs antigen production and virus titer. The exonuclease domains of ISG20 mainly participated in HBV-RNA decay. In vivo hydrodynamic injection, ISG20 was crucial for suppressing HBV replication without degrading host RNA in the liver. Taken together, ISG20 acts as an innate anti-HBV effector that selectively degrades HBV RNA and blocks replication of infectious HBV particles. ISG20 would be a critical effector for ameliorating chronic HBV infection in the IFN therapy.


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