Oncotarget

Research Papers:

Combination therapy with anti-ErbB3 monoclonal antibodies and EGFR TKIs potently inhibits Non-small Cell Lung Cancer

Alessia Noto, Claudia De Vitis, Giuseppe Roscilli, Luigi Fattore, Debora Malpicci, Emanuele Marra, Laura Luberto, Antonio D'Andrilli, Pierpaolo Coluccia, Maria Rosaria Giovagnoli, Nicola Normanno, Luigi Ruco, Luigi Aurisicchio, Rita Mancini and Gennaro Ciliberto _

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Oncotarget. 2013; 4:1253-1265. https://doi.org/10.18632/oncotarget.1141

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Abstract

Alessia Noto1,2, Claudia De Vitis2,3, Giuseppe Roscilli1,4, Luigi Fattore1,2, Debora Malpicci2, Emanuele Marra4, Laura Luberto3, Antonio D’Andrilli6, Pierpaolo Coluccia2, Maria Rosaria Giovagnoli1,6, Nicola Normanno7, Luigi Ruco1,6, Luigi Aurisicchio4,5, Rita Mancini1,2, Gennaro Ciliberto7

1 Dipartimento di Medicina Clinica e Molecolare, Sapienza Universita’ di Roma, Italy;

2 Dipartimento di Chirurgia “P. Valdoni”, Sapienza Università di Roma;

3 Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro “Magna Graecia”, Catanzaro, Italy

4 Takis s.r.l., Via di Castel Romano 100, 00128 Roma, Italy;

5 BIOGEM scarl, via Camporeale, Ariano Irpino (Av), Italy;

6 Azienda Ospedaliera S. Andrea, Roma, Italy;

7 IRCCS Istituto Nazionale Tumori “Fondazione G. Pascale”, Napoli, Italy

Correspondence:

Gennaro Ciliberto, email:

Keywords: Lung cancer, primary cultures, TKIs, ErbB3, monoclonal antibodies

Received: July 2, 2013 Accepted: July 19, 2013 Published: July 21, 2013

Abstract

Personalized therapy of advanced non-small cell lung cancer (NSCLC) has been improved by the introduction of EGFR tyrosine kinase inhibitors (TKIs), gefitinib and erlotinib. EGFR TKIs induce dramatic objective responses and increase survival in patients bearing sensitizing mutations in the EGFR intracytoplasmic tyrosine kinase domain. However, virtually all patients develop resistance, and this is responsible for disease relapse. Hence several efforts are being undertaken to understand the mechanisms of resistance in order to develop combination treatments capable to sensitize resistant cells to EGFR TKIs. Recent studies have suggested that upregulation of another member of the EGFR receptor family, namely ErbB3 is involved in drug resistance, through increased phosphorylation of its intracytoplasmic domain and activation of PI3K/AKT signaling. In this paper we first show, by using a set of malignant pleural effusion derived cell cultures (MPEDCC) from patients with lung adenocarcinoma, that surface ErbB3 expression correlates with increased AKT phosphorylation. Antibodies against ErbB3, namely A3, which we previously demonstrated to induce receptor internalization and degradation, inhibit growth and induce apoptosis only in cells overexpressing surface ErbB3. Furthermore, combination of anti-ErbB3 antibodies with EGFR TKIs synergistically affect cell proliferation in vitro, cause cell cycle arrest, up-regulate p21 expression and inhibit tumor growth in mouse xenografts. Importantly, potentiation of gefitinib by anti-ErbB3 antibodies occurs both in de novo and in ab initio resistant cells. Anti-ErbB3 mAbs strongly synergize also with the dual EGFR and HER2 inhibitor lapatinib. Our results suggest that combination treatment with EGFR TKI and antibodies against ErbB3 should be a promising approach to pursue in the clinic.


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