Oncotarget

Research Papers:

Spatial distribution of FoxP3+ and CD8+ tumour infiltrating T cells reflects their functional activity

Rebecca Posselt, Katharina Erlenbach-Wünsch, Matthias Haas, Jonas Jeßberger, Maike Büttner-Herold, Marlen Haderlein, Markus Hecht, Arndt Hartmann, Rainer Fietkau and Luitpold V. Distel _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2016; 7:60383-60394. https://doi.org/10.18632/oncotarget.11039

Metrics: PDF 702 views  |   HTML 981 views  |   ?  


Abstract

Rebecca Posselt1,*, Katharina Erlenbach-Wünsch2,*, Matthias Haas3, Jonas Jeßberger1, Maike Büttner-Herold4, Marlen Haderlein1, Markus Hecht1, Arndt Hartmann2, Rainer Fietkau1, Luitpold V. Distel1

1Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

2Institute of Pathology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

3Department of Radiology, Charité Universitätsmedizin, Berlin, Germany

4Deparment of Nephropathology, Institute of Pathology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

*These authors have contributed equally to this work

Correspondence to:

Luitpold V. Distel, email: Luitpold.Distel@uk-erlangen.de

Keywords: regulatory T cells, cytotoxic T cells, FoxP3+, CD8+, tumour-infiltrating lymphocytes

Received: May 10, 2016    Accepted: July 19, 2016    Published: August 03, 2016

ABSTRACT

Background: Regulatory and cytotoxic T cells are key players in the host’s anticancer immune response. We studied the spatial distribution of FoxP+ and CD8+ cells to identify potential interactions.

Methods: In 202 patients 103 pre-radiochemotherapy biopsies and 153 post-radiochemotherapy tumour specimens of advanced rectal cancer were available and an immunohistochemical double staining of FoxP3+ and CD8+ tumour-infiltrating lymphocytes was performed to investigate cell density and cell-to-cell distances.

Results: FoxP3+ cells decreased after radiochemotherapy by a factor of 3 while CD8+ cells remained nearly unchanged. High epithelial (p=0.033) and stromal (p=0.009) FoxP3+ cell density was associated with an improved overall survival. Cell-to-cell distances of randomly distributed cells were simulated and compared to observed cell-to-cell distances. Observed distances shorter than the simulated, random distances were hypothesized to represent FoxP3+ cells actively interacting with CD8+ cells. Epithelial short distances were associated with a favourable prognosis while the opposite was true for the stromal compartment.

Conclusion: The analysis of cell-to-cell distances may offer a tool to predict outcome, maybe by identifying functionally active, interacting infiltrating inflammatory cells in different tumour compartments.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 11039