Oncotarget

Research Papers:

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma

Yu You, Jia-Xin Tan, Hai-Su Dai, Hao-Wei Chen, Xue-Jun Xu, Ai-Gang Yang, Yu-Jun Zhang, Lian-Hua Bai and Ping Bie _

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Oncotarget. 2016; 7:57099-57116. https://doi.org/10.18632/oncotarget.10981

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Abstract

Yu You1, Jia-Xin Tan1, Hai-Su Dai1, Hao-Wei Chen1, Xue-Jun Xu1, Ai-Gang Yang1, Yu-Jun Zhang1, Lian-Hua Bai1, Ping Bie1

1Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China

Correspondence to:

Ping Bie, email: bieping@medmail.com.cn

Lian-Hua Bai, email: 870426131@qq.com

Keywords: hepatocellular carcinoma, hepatic stellate cells, galectin-1, miRNA-22

Received: April 14, 2016     Accepted: July 10, 2016     Published: August 1, 2016

ABSTRACT

Hepatic stellate cells (HSCs) induce immune privilege and promote hepatocellular carcinoma (HCC) by suppressing the immune system. On the other hand, galectin-1 and miRNA-22 (miR-22) are dysregulated in HCC and serve as prognostic indicators for patients. In this study, therefore, we measured galectin-1 and miR-22 expression in HSCs isolated from HCC tissues (Ca-HSCs), and in normal liver tissues (N-HSCs) as a control. We also investigated the apoptosis rate among T cells and the production of cytokines (IFN-γ and IL-10) in HSCs co-cultured with T cells. And we used immunohistochemical staining to tested for correlation between galectin-1 expression, CD3 expression and clinicopathological features in 162 HCC patients. Our results showed that galectin-1 expression was much higher in Ca-HSCs than in N-HSCs. Overexpression of galectin-1 promoted HSC-induced T cell apoptosis and cytokine production (IFN-γ and IL-10), while miR-22 expression inhibited it. Galectin-1 expression correlated negatively with miR-22 expression in HSCs. High galectin-1 and low CD3 expression levels were associated with poor prognosis in HCC patients. These results suggest that the immunosuppressive microenvironment promoted by HSC-derived galectin-1 in HCC can be inhibited by miR-22. Galectin-1 and miR-22 could potentially serve as prognostic markers and therapeutic targets in HCC.


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