Tumor exosome-mediated promotion of adhesion to mesothelial cells in gastric cancer cells

Tomohiro Arita; Daisuke Ichikawa; Hirotaka Konishi; Shuhei Komatsu; Atsushi Shiozaki; Shinpei Ogino; Yuji Fujita; Hidekazu Hiramoto; Junichi Hamada; Katsutoshi Shoda; Toshiyuki Kosuga; Hitoshi Fujiwara; Kazuma Okamoto; Eigo Otsuji

doi:10.18632/oncotarget.10869

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Tumor exosome-mediated promotion of adhesion to mesothelial cells in gastric cancer cells

Tomohiro Arita, Daisuke Ichikawa _, Hirotaka Konishi, Shuhei Komatsu, Atsushi Shiozaki, Shinpei Ogino, Yuji Fujita, Hidekazu Hiramoto, Junichi Hamada, Katsutoshi Shoda, Toshiyuki Kosuga, Hitoshi Fujiwara, Kazuma Okamoto and Eigo Otsuji

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2016; 7:56855-56863. https://doi.org/10.18632/oncotarget.10869

Metrics: PDF 3842 views | HTML 3810 views | ?

Abstract

Tomohiro Arita1, Daisuke Ichikawa1, Hirotaka Konishi1, Shuhei Komatsu1, Atsushi Shiozaki1, Shinpei Ogino1, Yuji Fujita1, Hidekazu Hiramoto1, Junichi Hamada1, Katsutoshi Shoda1, Toshiyuki Kosuga1, Hitoshi Fujiwara1, Kazuma Okamoto1, Eigo Otsuji1

1Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan

Correspondence to:

Daisuke Ichikawa, email: [email protected]

Keywords: gastric cancer, exosome, peritoneal metastasis, fibronectin 1, laminin gamma 1

Received: December 20, 2015 Accepted: July 18, 2016 Published: July 28, 2016

ABSTRACT

Background: Peritoneal metastasis consists of a highly complex series of steps, and the details of the underlying molecular mechanism remain largely unclear. In this study, the effects of tumor-derived exosomes (TEX) on the progression of gastric cancers were investigated in peritoneal metastasis.

Results: TEX were internalized in both mesothelial and gastric cancer cells in a cellular origin non-specific manner. Internalization of TEX into mesothelial cells promoted significant adhesion between mesothelial and gastric cancer cells, and TEX internalization into gastric cancer cells significantly promoted migratory ability, while internalization of mesothelial cell-derived exosomes did not. Expression of adhesion-related molecules, such as fibronectin 1 (FN1) and laminin gamma 1 (LAMC1), were increased in mesothelial cells after internalization of TEX from gastric cancer cell line and malignant pleural effusion.

Methods: TEX were extracted from cell-conditioned medium by ultracentrifugation. The effects of TEX on the malignant potential of gastric cancer were investigated in adhesion, invasion, and proliferation assays. PCR array as well as western blotting were performed to determine the underlying molecular mechanisms. The molecular changes in mesothelial cell after internalization of TEX derived from malignant pleural effusion were also confirmed.

Conclusions: TEX may play a critical role in the development of peritoneal metastasis of gastric cancer, which may be partially due to inducing increased expression of adhesion molecules in mesothelial cells.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 10869

Publication Alerts

Research Papers:

Tumor exosome-mediated promotion of adhesion to mesothelial cells in gastric cancer cells

Abstract