Loss of ZFP36 expression in colorectal cancer correlates to wnt/ β-catenin activity and enhances epithelial-to-mesenchymal transition through upregulation of ZEB1, SOX9 and MACC1
Metrics: PDF 884 views | HTML 869 views | ?
Lucia Montorsi1, Filippo Guizzetti1, Claudia Alecci1, Andrea Caporali2, Andrea Martello2, Claudio Giacinto Atene1, Sandra Parenti1, Silvia Pizzini5, Paola Zanovello4, Stefania Bortoluzzi3, Sergio Ferrari1, Alexis Grande1,*, Tommaso Zanocco-Marani1,*
1Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy
2University/BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK
3Department of Molecular Medicine, University of Padova, Padova, Italy
4Department of Surgery, Oncology and Gastroenterology (DiSCOG), University of Padova, Padova, Italy
5Centre for Integrative Biology (CIBIO), University of Trento, Povo (Trento), Italy
*These authors contributed equally to this work
Tommaso Zanocco-Marani, email: firstname.lastname@example.org
Keywords: ZFP36, tristetraprolin, colon cancer, β-catenin, epithelial mesenchymal transition
Received: March 21, 2016 Accepted: July 09, 2016 Published: July 24, 2016
The mRNA-destabilizing protein ZFP36 has been previously described as a tumor suppressor whose expression is lost during colorectal cancer development. In order to evaluate its role in this disease, we restored ZFP36 expression in different cell contexts, showing that the presence of this protein impairs the epithelial-to-mesenchymal transition (EMT) and induces a higher susceptibility to anoikis. Consistently, we found that ZFP36 inhibits the expression of three key transcription factors involved in EMT: ZEB1, MACC1 and SOX9. Finally, we observed for the first time that its expression negatively correlates with the activity of Wnt/β-catenin pathway, which is constitutively activated in colorectal cancer. This evidence provides a clue on the mechanism leading to the loss of ZFP36 in CRC.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.