POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer

Daniel J. Sharpe; Katy S. Orr; Michael Moran; Sharon J. White; Stephen McQuaid; Terence R. Lappin; Alexander Thompson; Jacqueline A. James

doi:10.18632/oncotarget.2492

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer

Daniel J. Sharpe, Katy S. Orr, Michael Moran, Sharon J. White, Stephen McQuaid, Terence R. Lappin, Alexander Thompson and Jacqueline A. James _

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2014; 5:8803-8815. https://doi.org/10.18632/oncotarget.2492

Metrics: PDF 2818 views | HTML 2904 views | ?

Abstract

Daniel J. Sharpe1, Katy S. Orr1, Michael Moran1, Sharon J. White2, Stephen McQuaid3, Terence R. Lappin1, Alexander Thompson1 and Jacqueline A. James1,3

1 Centre for Cancer Research and Cell Biology, Queen’s University Belfast, Belfast

2 Pathology Department, Ninewells Hospital & Medical School, Dundee

3 The Northern Ireland Molecular Pathology Laboratory, Centre for Cancer Research and Cell Biology, Queen’s University Belfast, Belfast and The Belfast Trust, Belfast City Hospital, Lisburn Road, Belfast

Correspondence:

Jacqueline A James, email:

Keywords: Head and Neck cancer; HOXD10; HOXD11; POU2F1; Biomarker

Received: July 16, 2014 Accepted: September 15, 2014 Published: September 16, 2014

Abstract

HOX genes are master regulators of organ morphogenesis and cell differentiation during embryonic development, and continue to be expressed throughout post-natal life. To test the hypothesis that HOX genes are dysregulated in head and neck squamous cell carcinoma (HNSCC) we defined their expression profile, and investigated the function, transcriptional regulation and clinical relevance of a subset of highly expressed HOXD genes.

Two HOXD genes, D10 and D11, showed strikingly high levels in HNSCC cell lines, patient tumor samples and publicly available datasets. Knockdown of HOXD10 in HNSCC cells caused decreased proliferation and invasion, whereas knockdown of HOXD11 reduced only invasion.

POU2F1 consensus sequences were identified in the 5’ DNA of HOXD10 and D11. Knockdown of POU2F1 significantly reduced expression of HOXD10 and D11 and inhibited HNSCC proliferation. Luciferase reporter constructs of the HOXD10 and D11 promoters confirmed that POU2F1 consensus binding sites are required for optimal promoter activity.

Utilizing patient tumor samples a significant association was found between immunohistochemical staining of HOXD10 and both the overall and the disease-specific survival, adding further support that HOXD10 is dysregulated in head and neck cancer. Additional studies are now warranted to fully evaluate HOXD10 as a prognostic tool in head and neck cancers.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 2492

Publication Alerts

Research Papers:

POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer

Abstract