An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

Zhi Shan; Xianfeng Lin; Shengyu Wang; Xuyang Zhang; Yichuan Pang; Shengyun Li; Tianming Yu; Shunwu Fan; Fengdong Zhao

doi:10.18632/oncotarget.16831

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Research Papers:

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

Zhi Shan, Xianfeng Lin, Shengyu Wang, Xuyang Zhang, Yichuan Pang, Shengyun Li, Tianming Yu, Shunwu Fan and Fengdong Zhao _

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Oncotarget. 2017; 8:40276-40288. https://doi.org/10.18632/oncotarget.16831

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Abstract

Zhi Shan1,*, Xianfeng Lin1,*, Shengyu Wang1,*, Xuyang Zhang1,*, Yichuan Pang2, Shengyun Li1, Tianming Yu1, Shunwu Fan1 and Fengdong Zhao1

1Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China

2MOE Key Laboratory of Macromolecular synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China

*These authors are contributed equally to this work

Correspondence to:

Fengdong Zhao, email: [email protected]

Shunwu Fan, email: [email protected]

Keywords: intervertebral disc degeneration, small intestinal submucosa, nucleus pulposus cell, decellularization, extracellular matrix

Received: July 13, 2016 Accepted: February 27, 2017 Published: April 04, 2017

ABSTRACT

We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix. The xenogeneic decellularized SIS did not display cytotoxicity in vitro and its application prevented NPC degradation. Furthermore, the xenogeneic SIS microparticles were effective in preventing IVD progression in vivo in a rabbit disc degeneration model. In conclusion, our study describes an optimized method for decellularized SIS preparation and demonstrated that the material is safe and effective for treating IVD degeneration.

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Research Papers:

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

Abstract