ED-B fibronectin expression is a marker of epithelial-mesenchymal transition in translational oncology

Iacopo Petrini; Serena Barachini; Vittoria Carnicelli; Sara Galimberti; Letizia Modeo; Roberto Boni; Martina Sollini; Paola Anna Erba

doi:10.18632/oncotarget.13615

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

ED-B fibronectin expression is a marker of epithelial-mesenchymal transition in translational oncology

Iacopo Petrini _, Serena Barachini, Vittoria Carnicelli, Sara Galimberti, Letizia Modeo, Roberto Boni, Martina Sollini and Paola Anna Erba

PDF | HTML | How to cite

Oncotarget. 2017; 8:4914-4921. https://doi.org/10.18632/oncotarget.13615

Metrics: PDF 2316 views | HTML 2696 views | ?

Abstract

Iacopo Petrini1, Serena Barachini2, Vittoria Carnicelli3, Sara Galimberti2, Letizia Modeo4, Roberto Boni4, Martina Sollini5, Paola Anna Erba4

1General Pathology, Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy

2Laboratory of Hematology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

3Biochemistry, Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy

4Nuclear Medicine, Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy

5Department of Biomedical Sciences, Humanitas University, Rozzano, Milan, Italy

Correspondence to:

Iacopo Petrini, email: [email protected]

Keywords: fibronectin, endothelial-to-mesenchymal transition, ED-B, prostate cancer, TGF-β

Received: July 25, 2016 Accepted: November 09, 2016 Published: November 25, 2016

ABSTRACT

Fibronectin is a component of the extracellular matrix that links collagen fibers to integrins on the cell’s surface. The splicing isoforms, containing the ED-B domain, are not expressed in adult tissues but only in tumor stroma or during embryonic development. Fibroblasts and endothelial cells express ED-B fibronectin during angiogenesis. Also cancer cells can synthetize ED-B fibronectin, but its function in tumor growth needs to be further elucidated.

We evaluated the expression of ED-B fibronectin in prostate cancer cell lines: PC3 and DU145. Using TGF-β, we induced epithelial to mesenchymal transition in culture and observed an increase of ED-B fibronectin expression. Thereafter, we evaluated the expression of ED-B fibronectin in multipotent mesangiogenic progenitor cells, and in mesenchymal stromal cells. The expression of ED-B fibronectin was much higher in mesenchymal than prostate cancer cells even after the epithelial to mesenchymal transition.

Epithelial to mesenchymal transition is a key step for tumor progression contributing to the metastatic spread. Therefore, circulating cancer cells could seed into the metastatic niche taking advantage from the ED-B fibronectin that secrete their own.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 13615

Publication Alerts

Research Papers:

ED-B fibronectin expression is a marker of epithelial-mesenchymal transition in translational oncology

Abstract