Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells

Vera Dugina; Irina Alieva; Natalya Khromova; Igor Kireev; Peter W. Gunning; Pavel Kopnin

doi:10.18632/oncotarget.12236

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Research Papers:

Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells

Vera Dugina, Irina Alieva, Natalya Khromova, Igor Kireev, Peter W. Gunning and Pavel Kopnin _

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Oncotarget. 2016; 7:72699-72715. https://doi.org/10.18632/oncotarget.12236

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Abstract

Vera Dugina1,3, Irina Alieva1,3, Natalya Khromova2, Igor Kireev1, Peter W. Gunning3 and Pavel Kopnin2

1 Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia

2 Blokhin Russian Cancer Research Center, Moscow, Russia

3 School of Medical Science, The University of New South Wales, NSW, Sydney, Australia

Correspondence to:

Pavel Kopnin, email:

Keywords: EB1, +TIPs, microtubules, actin isoforms, β-actin, γ-actin

Received: September 9, 2016 Accepted: September 18, 2016 Published: September 24, 2016

Abstract

Actin microfilaments and microtubules are both highly dynamic cytoskeleton components implicated in a wide range of intracellular processes as well as cell-cell and cell-substrate interactions. The interactions of actin filaments with the microtubule system play an important role in the assembly and maintenance of 3D cell structure. Here we demonstrate that cytoplasmic actins are differentially distributed in relation to the microtubule system. LSM, 3D-SIM, proximity ligation assay (PLA) and co-immunoprecipitation methods applied in combination with selective depletion of β- or γ-cytoplasmic actins revealed a selective interaction between microtubules and γ-, but not β-cytoplasmic actin via the microtubule +TIPs protein EB1. EB1-positive comet distribution analysis and quantification have shown more effective microtubule growth in the absence of β-actin. Our data represent the first demonstration that microtubule +TIPs protein EB1 interacts mainly with γ-cytoplasmic actin in epithelial cells.

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Research Papers:

Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells

Abstract