Reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1

Previous studies indicated that miR-200s participated in IL-6-induced hepatic insulin resistance. However, the role of miR-200s in hepatic lipid accumulation has not been elucidated. Here we found that miR-200b and miR-200c were reduced in the steatotic livers of mice fed a high-fat diet (HFD) and patients with nonalcoholic fatty liver disease. This down-regulation was accompanied by an increase in the expression of lipogenic proteins such as sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS). The suppression of miR-200b and miR-200c in Hep1-6 and NCTC1469 hepatocytes enhanced intracellular triglyceride levels, which were associated with increased SREBP-1 and FAS protein levels. In contrast, the over-expression of miR-200b and miR-200c suppressed lipid accumulation and reduced the expression of SREBP1 and FAS in Hep1-6 and NCTC1469 cells transfected with miR-200b or miR-200c mimics. Importantly, the up-regulation of miR-200b and miR-200c could reverse oleic acid/palmitic acid-induced lipid accumulation in hepatocytes. A luciferase reporter assay identified that miR-200b and miR-200c could directly bind the 3′UTR of jun. JUN activated the transcription of srebp1 to increase lipid accumulation. The data also demonstrated that increased miR-200b and miR-200c expression might be associated with sitagliptin-reduced hepatic lipid accumulation in mice fed a HFD. These findings suggest, for the first time, that reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1.

MicroRNAs (miRs) are small, non-coding RNAs that are important post-transcriptional regulators of gene expression. By binding to the 3′ untranslated region (3′UTR) of their target genes, they globally repress gene expression [14]. miRs have been shown to be involved in a wide variety of physiological and psychological processes [15,16]. miR-34a was increased in patients with NAFLD and in mice fed a high-fat diet (FHDfed mice) [17]. miR-33a and miR-33b were found to globally regulate lipid metabolism by targeting numerous lipid metabolism-associated genes including abca1, crot, cpt1a, hadhb and ampkα [19]. The miR-200 family, including miR-200a, miR-200b, miR-200c, miR-141 and miR-429, has been reported to be dysregulated in several types of cancers including gastric cancer, breast cancer and bladder cancer [20][21][22]. In a previous study, we demonstrated that the reduction of miR-200a, miR-200b and miR-200c in hepatocytes contributed to IL-induced insulin resistance [23]. However, the role of the miR-200 family in hepatic lipid metabolism remains unknown. The aim of this study was to explore the potential role of the miR-200 family in the regulation of hepatic lipid metabolism. The findings suggest that the reduced expression of miR-200b and miR-200c participated in abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1.

rEsULts the levels of mir-200b and mir-200c are reduced in the steatotic livers of HFD-fed mice and NAFLD patients
Oil red O and H&E staining showed abnormal lipid accumulation in the livers of mice fed a HFD ( Figure 1A). The livers of HFD-fed mice displayed excessive amounts of triglycerides ( Figure 1B). Moreover, the expression of lipogenic genes including srebp1 and fas was significantly increased in the livers of HFD-fed mice ( Figure 1C). To identify the potential role of the miR-200 family in lipid metabolism, relative expression patterns were analyzed in the steatotic livers of HFD-fed mice and NAFLD patients. As shown in Figure 1D, the levels of miR-200b and miR-200c, but not the levels of other members of the miR-200 family including miR-200a, miR-141 and miR-429, were obviously reduced in the livers of HFD-fed mice. As shown in Table 1, the age and gender distribution were similar between the healthy controls and NAFLD patients. Characteristics such as BMI, waist circumference and triglyceride levels were significantly higher in the NAFLD patients compared with the healthy controls. H&E staining showed that the cytoplasm of the NAFLD patient hepatocytes was filled with lipid droplets ( Figure 1E). Importantly, the expression of miR-200b and miR-200c was suppressed in the livers of NAFLD patients ( Figure 1F), and the levels of lipogenic proteins such as SREBP1 and FAS were elevated compared with the healthy controls ( Figure 1G). These data suggest that miR-200b and miR-200c may be involved in hepatic lipogenesis.

reduced mir-200b and mir-200c expression contributes to abnormal lipid accumulation in Hep1-6 and Nctc1469 cells
To determine whether miR-200b and miR-200c are involved in abnormal hepatic lipid accumulation, the expression of miR-200b and miR-200c was suppressed in two murine liver cell lines, Hep1-6 and NCTC1469, by transfection with miR-200b and miR-200c inhibitors. As shown in Figure 2A and 2B, the transfection of both Hep1-6 and NCTC1469 cells with miR-200b and miR-200c inhibitors resulted in a significant increase in intracellular triglyceride levels. This in turn was associated with elevated SREBP-1 and FAS protein levels ( Figure 2C and 2D). This finding suggests that reduced miR-200b and miR-200c expression might contribute to abnormal lipid accumulation in hepatocytes. In contrast, the over-expression of miR-200b and miR-200c significantly reduced lipid accumulation in Hep1-6 and NCTC1469 cells transfected with miR-200b and miR-200c mimics ( Figure 2E and 2F). This was accompanied by decreased SREBP1 and FAS levels ( Figure 2G and 2H), indicating that miR-200b and miR-200c mimics have a suppressive role in lipid accumulation.

the over-expression of mir-200b and mir-200c reverses oleic acid/palmitic acid-induced lipid accumulation in hepatocytes
To further investigate the suppressive role of miR-200b and miR-200c mimics in lipid accumulation, Hep1-6 and NCTC1469 cells were pre-treated with a mixture of oleic acid and palmitic acid (2:1, M/M) for 24 h. Oil red O staining revealed that pre-treatment with oleic acid/palmitic acid (O/P) significantly promoted lipid accumulation in Hep1-6 and NCTC1469 cells ( Figure 3A and 3B). Interestingly, the transfection of both Hep1-6 and NCTC1469 cells with miR-200b and miR-200c mimics partially reversed the formation of the O/P-induced lipid droplets ( Figure 3A and 3B) and the elevation of SREBP1 and FAS levels ( Figure 3C and 3D).

jun is a target gene of mir-200b and mir-200c
To explore the specific mechanism by which miR-200b and miR-200c regulate lipogenesis in hepatocytes, miR-200b and miR-200c target genes were identified through the bioinformatics websites miRBase, TargetScan and RNA22. Unfortunately, no target genes directly involved in lipid metabolism were identified. Instead, a transcription factor, jun, was predicted to be a target gene of miR-200b and miR-200c ( Figure 4A). To verify this finding, the 3′UTR of jun was cloned into the pmirGLO plasmid. As shown in Figure 4B, the miR-200b and miR-200c mimics significantly reduced the relative luciferase activity of pmirGLO-jun-3′UTR, indicating a direct binding. Furthermore, the mutant of jun 3′UTR was inserted into the pmirGLO plasmid, but overexpression of miR-200b and miR-200c could not decrease the relative luciferase activity of pmirGLO-jun-3′UTR-Mut ( Figure 4B). Western blotting showed that the over-expression of miR-200b and miR-200c obviously reduced the level of JUN protein ( Figure 4C), while the inhibition of miR-200b and miR-200c significantly elevated the expression of JUN in Hep1-6 cells ( Figure 4D), demonstrating that jun is a target gene of miR-200b and miR-200c.

JUN stimulates lipid accumulation by activating the transcription of srebp1
As a transcription factor, JUN participates in cell proliferation, cell survival, apoptosis and tumorigenesis by activating gene expression [24,25]. In the present study, two putative JUN binding sites were identified in the promoter region of the mouse srebp1 gene. To confirm the potential binding sites, the regions of the srebp1 promoter containing both of the binding sites, one of the binding sites (pGL3-srebp1-S1) or neither of the binding sites (pGL3-srebp1-S2) were cloned into the pGL3 vector ( Figure 5A). HEK293 cells were then transfected with siRNA specifically targeting jun (si-jun-1 or si-jun-2) along with the luciferase reporter plasmids containing srebp1, srebp1-S1 or srebp1-S2. The results indicated that the knockdown of jun decreased the relative luciferase activity of pGL3-srebp1 and pGL3-srebp1-S1, but had no effect on pGL3-srebp1-S2 ( Figure 5B). Furthermore, a ChIP assay revealed that the knockdown of jun in Hep1-6 cells reduced the binding between JUN and the srebp1 promoter, while the over-expression of JUN enhanced it ( Figure 5C). The knockdown of jun reduced SREBP1 and FAS protein levels ( Figure 5D and 5E) as well as lipid accumulation ( Figure 5F) in both Hep1-6 and NCTC1469 cells. More importantly, the knockdown of jun could partially ameliorate the miR-200b and miR-200c inhibition-induced increased SREBP1 and FAS levels in Hep1-6 cells ( Figure 5G). Taken together, these data suggest that miR-200b and miR-200c are involved in lipogenesis through stimulation of JUN expression and the subsequent activation of srebp1 transcription.  Figure 6A). More importantly, this enhancement of hepatic miR-200b and miR-200c expression led to a significant reduction in liver weight and in the liver weight-to-body weight ratio ( Figure 6B, 6C). H&E and Oil Red O staining revealed decreased hepatic lipid deposition in these animals ( Figure 6D). Of note, the hepatic triglyceride content was significantly reduced ( Figure 6E), and this was associated with decreased levels of JUN, SREBP-1 and FAS protein in the liver ( Figure 6F). These results suggest that the up-regulation of miR-200b and miR-200c expression in the liver may prevent the accumulation of hepatic triglycerides in HFD-fed mice by suppressing JUN-SREBP1-stimulated lipogenesis.

Elevated mir-200b and mir-200c expression is associated with sitagliptin-reduced hepatic lipid accumulation in HFD-fed mice
Studies have suggested that sitagliptin reduces hepatic lipid accumulation [26,27]. However, the specific mechanism has not been fully elucidated. In the present study, Hep1-6 cells were pre-treated with  Figure 7A). The study was further extended to HFD-fed mice. Interestingly, miR-200b and miR-200c were enhanced in the livers of HFD-fed mice treated with sitagliptin ( Figure 7B). Oil red O and H&E staining showed that hepatic accumulation was improved when HFD-fed mice were administered 3 mg/ kg/day sitagliptin via i.g. for 8 weeks compared with the vehicle control ( Figure 7C). Hepatic triglyceride contents ( Figure 7D) and SREBP1 expression levels ( Figure 7E) were also reduced in sitagliptin-treated, HFD-fed mice. These data indicated that an increase in miR-200b and miR-200c expression might be associated with the sitagliptin-induced reversal of hepatic lipid accumulation in HFD-fed mice.
To further assess the requirement of miR-200 in sitagliptin's effect, miR-200b and miR-200c were knocked down in the Hep1-6 cells pre-treated with a mixture of oleic acid/palmitic acid and treated with sitagliptin. We found that sitagliptin treatment significantly decreased the expression of SREBP1 and FAS. More importantly, we demonstrated that down-regulation of miR-200b and miR-200c could partially reverse sitagliptin-induced decreased levels of SREBP1 and FAS, and reduced contents of intracellular triglyceride in Hep1-6 cells ( Figure 7F, 7G).   inhibitors. The data represent the mean ± SEM of three independent experiments. *P < 0.05 and **P < 0.01 versus the control. www.impactjournals.com/oncotarget DIscUssION NAFLD is widely recognized as the most common cause of liver damage. It is characterized by abnormal fat accumulation in the liver and may eventually progress to nonalcoholic steatohepatitis (NASH) with progressive fibrosis [28]. Although great advances have been made in elucidating the pathogenesis of NAFLD, the underlying mechanism is still largely unknown. Emerging evidence suggests that miRNAs are involved in the signaling pathways of NAFLD. Previously, we examined miRNA expression in the livers of db/db mice and found that the expression of the miR-200 family members miR-200a, miR-200b and miR-200c was reduced in response to hepatic insulin resistance [23]. The miR-200 family has been extensively studied in different tumors [29][30][31]. Researchers demonstrated that the miR-200 family influences cancer development and progression by targeting various important signaling factors. However, few studies have been conducted on metabolic disorders. Our previous study demonstrated that the down-regulation of miR-200s following treatment with IL-6 could reduce the activation of the PI3K/AKT/GSK signaling pathway by targeting FOG2 [23]. In this study, we found that only miR-200b and miR-200c were decreased in the livers of NAFLD patients and mice fed a HFD. Additional experiments conducted in Hep1-6 and NCTC1469 cells revealed that the reduced expression of miR-200b and miR-200c led to abnormal lipid accumulation in hepatocytes, whereas the up-regulation of miR-200b and Results of the luciferase reporter assay conducted in HEK293T cells co-transfected with siRNA specifically inhibiting jun (si-jun-1 or si-jun-2) and a luciferase reporter plasmid containing the promoters of either srebp1 (pGL3-srebp1), srebp1-S1 (pGL3-srebp1-S1) or srebp1-S2 (pGL3-srebp1-S2).   In this manuscript, we identified jun as a target gene of miR-200b and miR-200c. Bioinformatics predictions revealed that there were conserved miR-200b and miR-200c binding sites in the 3′UTR of jun. The presence of these sites was validated using a luciferase reporter assay. JUN is correlated with a variety of biological processes including cell survival, cell proliferation, apoptosis and development [24,33]. Through its transcriptional activity, JUN controls the expression of numerous genes involved in a variety of physiological processes. As a member of the basic leucine zipper motif (bZIP) family of proteins, JUN contains a basic DNAbinding domain and a leucine zipper region [34,35]. JUN-protein interactions comprise a complex regulatory network that functions in a cell type-sepcific manner [36]. In the present study, we identified two putative JUN sites TGCGTCA and TGACTCA [37] in the promoter region of murine srebp1, raising the possibility that JUN may bind to the srebp1 promoter. The results of a luciferase reporter assay showed that the knockdown of jun significantly reduced the relative luciferase activity of the srebp1 promoter. Furthermore, the suppression of jun gene obviously reduced the expression of SREBP1, indicating that JUN activates SREBP1 expression.
Sitagliptin, a dipeptidyl peptidase (DPP)-4 inhibitor, was found to maintain glucose homeostasis by reducing the inactivation of incretin hormones such as glucagon-like peptide-1 (GLP-1) [40]. In vitro studies demonstrated that GLP-1 could enhance the activation of adenosine monophosphate kinase and peroxisome proliferator-activated receptor-α, thereby inhibiting lipogenesis and increasing lipid oxidation, respectively [41,42]. In this study, we provide preliminary evidence that the elevated expression of miR-200b and miR-200c might contribute to the sitagliptin-induced reduction of hepatic lipid accumulation in HFD-fed mice. The treatment of HFD-fed mice with sitagliptin significantly impaired hepatic lipid accumulation and reduced the expression of SREBP1. Interestingly, the levels of miR-200b and miR-200c were increased in the livers of HFD-fed mice treated with sitagliptin. Moreover, knock down of miR-200b and miR-200c could partially rescue sitagliptin-induced reduced levels of SREBP1 and FAS, and decreased contents of intracellular triglyceride in Hep1-6 cells In conclusion, our findings may shed light on the specific molecular mechanism by which miR-200b and miR-200c are correlated with hepatic lipid metabolism.
The reduced expression of miR-200b and miR-200c contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1.

Experimental animals
Five-week-old male C57BL/6J mice were purchased from the Peking University Health Science Center. The C57BL/6J mice were fed a standard chow diet or a high-fat diet (HFD, D12451, 45% kcal from fat, Research Diet, USA, http://www.researchdiets.com/ opensource-diets/stock-diets) for 10 weeks starting at approximately 5 weeks of age. The mice were housed in a temperature-(20-24°C) and humidity-controlled (45-55%) environment with a 12 h/12 h light/dark cycle. Ten weeks after the initiation of the HFD, the C57BL/6J mice were treated with 3 mg/kg/day sitagliptin via i.g. for 8 weeks. These animals were sacrificed, and liver tissues were collected for further analysis.
All of the mouse procedures were approved by the Animal Ethics Committee at the Beijing Hospital.

Human liver specimens
The human liver biopsies and clinical procedures were performed with patient consent within the diagnostic workup of NAFLD. A total of 11 patients with NAFLD and 11 healthy control subjects were included in the study. Physical examinations, biochemical measurements, and body mass index (BMI) calculations were performed on all of the study participants. The application for patientderived materials was approved by the Research Ethics Committee of Beijing You-An Hospital, and written consent was obtained from all of the patients.

cell culture and treatment
The NCTC1469 murine liver cell line and the Hep1-6 hepatic cancer cell line were purchased from American Type Culture Collection. Both of the cell lines were cultured in low-glucose Dulbecco's modified Eagle medium (DMEM) (Invitrogen, Carlsbad, California, USA) supplemented with 10% horse serum (Hyclone, Logan, Utah, USA) or 10% fetal bovine serum (Gibico, Grand Island, New York, USA), 100 units/ml penicillin (Invitrogen, Carlsbad, California, USA) and 0.1 mg/ml streptomycin (Hyclone, Logan, Utah, USA) at 37°C with humidified air and 5% CO 2 . Sitagliptin (Sigma-Aldrich, St. Louis, Missouri, USA) was used at a final concentration of 1 μM.

Oleic acid/palmitic acid (O/P) treatment
0.25 M oleic acid (Sigma-Aldrich, St. Louis, Missouri, USA) and palmitic acid (Sigma-Aldrich, St. Louis, Missouri, USA) were dissolved in 100% ethyl alcohol. Before use, the 0.25 M oleic acid, the palmitic acid stock and 5% BSA were incubated in a 60°C water bath for 10 min. Then, 640 μl of the oleic acid or 320 μl of the palmitic acid stock were added drop-wise to 20 ml of 5% BSA to obtain 8 mM oleic acid and 4 mM palmitic acid, respectively. Before the experiments commenced, the 8 mM oleic acid and the 4 mM palmitic acid were incubated in a 60°C water bath for 10 min. Equal volumes of oleic acid and palmitic acid were mixed together. 300 μM oleic acid/palmitic acid mixture (2:1, M/M) was used to treat Hep1-6 and NCTC1469 cells for 24 h.

rNA isolation and real-time reversetranscription Pcr
The miRNAs were isolated using TRIzol reagent (Invitrogen, Carlsbad, California, USA) according to the manufacturer's instructions. To detect and quantify the miRNAs, stem-loop reverse-transcription polymerase chain reaction (RT-PCR) was performed as previously reported [44]. The specific primers used in this study were described previously [23].

Western blot analysis
The cells or liver tissues were lysed with RIPA buffer (Solarbio, Beijing, China). Fifteen µg of protein was separated by 10% SDS-PAGE and further transferred onto a PVDF membrane (Millipore, Boston, Massachusetts, USA). The membrane was blocked with 8% non-fat dry milk and incubated with primary antibodies at 4°C overnight. The specific primary antibodies used in this study were anti-SREBP1 (sc-366) (Santa Cruz, Dallas, Texas, USA), anti-FAS (#3180) (Cell Signaling, Boston, Massachusetts, USA), anti-β-actin (#3700) (Cell Signaling, Boston, Massachusetts, USA) and anti-JUN (#9165) (Cell Signaling, Boston, Massachusetts, USA). After washing three times with PBST, the membrane was incubated with the HRP-conjugated antirabbit or anti-mouse secondary antibody (Zhongshanjinqiao, Beijing, China. 1:5000) for 2 h at room temperature. Immunodetection was conducted using the ECL Plus detection system (Millpore, Boston, Massachusetts, USA) according to the manufacturer's instructions.

construction of adenovirus vectors
The adenovirus vector containing jun (Ad-jun, VH836318) and the control vector (Ad-NC, cp0001) were purchased from ViGene BioSciences (Rockville, Maryland, USA). And the adenovirus vector expressing mimics of miR-200b or miR-200c was constructed by Genechem (Shanghai, China).

chromatin immunoprecipitation (chIP)
The Chromatin Immunoprecipitation Assay Kit was purchased from Millipore (Boston, Massachusetts, USA). Briefly, the nuclei extracted from cells transfected with Ad-JUN/NC or si-JUN/NC sonicated into 200-1000 bp. Precleared chromatin was immunoprecipitated with anti-JUN and normal IgG antibodies according to the manufacturer's instructions. Immunocomplexes were added into 50 μl of protein A/G-Sepharose beads and purified with Qiaquick (QIAGEN, Duesseldorf, German) PCR purification columns. The precipitated DNA was amplified with srebp1-specific primers. The primers specific to the JUN binding sites on the srebp1 promoter were 5′-GTACCCTCAGGTCATACTGC-3′ and 5′-AAGCTCTCTGGATTGCCTT-3′.

Oil red O staining
Cells were treated with a mixture of oleic acid/ palmitic acid (O/P, 2:1, M/M) for 24 h and washed three times with PBS (5 min each). The cells were then fixed with 4% paraformaldehyde for 10 min at room temperature. After dehydration with 100% 1,2-propanediol for 5 s, the cells were stained with pre-warmed Oil Red O for 30 min at 37°C and washed with 85% 1,2-propanediol. The cells were washed with distilled water and observed under a microscope.

triglyceride measurement
The triglycerides in liver tissues or cells were extracted according to a modified method from Folch et al. [45]. Briefly, snap-frozen liver tissues (approximately 20 mg) were homogenized in 5 ml of 1 M NaOH and extracted with 5 ml of a chloroform/methanol (v/v = 2:1) solution. The organic layer was dried under nitrogen gas and resolubilized in 1 ml of chloroform containing 1% Triton X-100. The extract was dried again and resuspended in 1 ml of water. The triglyceride concentration was measured using a triglyceride enzymatic assay kit (ShenSuoYouFu Medical Diagnostic Products Co., Ltd., Shanghai, China).

Histological analysis of tissues
The liver samples were fixed in O.C.T. compound (Tissue-Tek, Japan) and sectioned at a thickness of 8 µm. The slides were stained with hematoxylin & eosin (H&E) or with Oil Red O as previously described [46,47].
Before the luciferase reporter assay was carried out, 5 × 10 4 cells in 500 μl of L-DMEM were seeded in each well of a 24-well plate and cultured for 18 h. The cells were transfected with the modified firefly luciferase vector (500 ng/μl) mixed with the Vigofect transfection reagent (Beijing, China) according to the manufacturer's instructions. After 48 h, the luciferase activities were measured using the dual-luciferase reporter assay system (Promega, Madison, Wisconsin, USA). Renilla luciferase activity was used as a normalization control.

Promoter reporter analysis
The PGL3 promoter vector and the PRL-TK vector were purchased from Promega Corporation. The promoter region of srebp1 was amplified from the genomic DNA of Hep1-6 cells. The PGL3 promoter vector and the amplified fragments were digested with XhoI/KpnI and purified by gel electrophoresis. The digested fragment was then inserted into the PGL3 vector up-stream of the SV40 promoter. HEK293T cells were co-transfected with the PGL3 plasmids and the PRL-TK vector using the VigoFect Transfection Reagent (Beijing, China). The cells were harvested and lysed 48 h posttransfection. The relative light units (RLU) were determined using the Dual-luciferase reporter assay system (Promega, Madison, Wisconsin, USA) according to the standard protocols. Normalized luciferase data (firefly/renilla) was compared with the empty pGL3promoter vector.