Analysis and application of the SRBSDV P 9-1 octamer crystal structure for the target of α-amino phosphonate derivatives

Southern rice black-streaked dwarf virus (SRBSDV) P9-1 octameric protein accumulates viroplasms in SRBSDV-infected plant and insect cells, our previous studies found α-amino phosphonate drug—dufulin had a micromole affinity with SRBSDV P9-1. Now we focus our studies on the SRBSDV P9-1 crystal structure and use it as the target for α-amino phosphonate derivatives. The structure of the SRBSDV P9-1 cylindrical octamer was determined to a 2.2 Å resolution using X-ray crystallography, this structure was composed of nine α-helices, nine β-sheets and a series of interconnecting loops. The structures of all eight subunits were nearly identical, except for some differences in the β-sheet core. There were six different sites (R20K, V109D, F164T, V123L, C124L and V128T) present between the SRBSDV P9-1 and the previously reported RBSDV P9-1 crystal structure. Fluorescence titration, isothermal calorimetry and microscale thermophoresis experiments showed that α-amino phosphonate derivatives GUFCC-013 and GUFCC-023 bound to SRBSDV P9-1 with micromole binding affinities. These results will provide important help for the further improvement of the lead compound and develop the potential anti-SRBSDV


INTRODUCTION
The formation of viroplasms (Vps) is a feature of the genus fijivirus.This formation is intrinsic to viral particle functioning in virus-infected plants and insect cells [1][2][3].Southern rice black-streaked dwarf virus (SRBSDV), a newly discovered member of the genus fijivirus [4,5], causes the devastating southern rice black-streaked dwarf and maize rough dwarf diseases in crops, which lead to severe yield losses in crops in China and Southeast Asia [6,7].The virus is transmitted by an insect vector, the white-backed planthopper sogatella furcifera (Hemiptera: delphacidae) [8].The genomic segment of SRBSDV is similar to rice black-streaked dwarf virus (RBSDV) and Mal de Río Cuarto virus (MRCV) [9][10][11].
Nonstructural virus proteins are required for the formation of cytoplasmic structures, and are regarded as key players in viral morphogenesis.In orbiviruses such as bluetongue virus, nonstructural protein NS2 is responsible for recruiting both the core proteins and newly synthesized transcripts [20].In phytoreoviruses such as rice dwarf virus, nonstructural protein Pns12 forms Vp-like structures [21].In rotaviruses encoded two nonstructural proteins, NSP2 and NSP5 [22], however, only NSP2 contributes to the binding of single-stranded RNA (ss-RNA) [23].Inmammalian genera of the family reoviridae, μNS is sufficient to form VFs (viral factories) [24], and it recruits σNS to VFs [27].The μNS protein acts as a scaffold to recruit viral core surface proteins [25], while σNS binds preferentially to ss-RNA and ss-DNA [26,27].In members of the genus fijivirus such as RBSDV, nonstructural protein RBSDV P9-1 is important for the functioning of the relative proteins in Vps during viral morphogenesis [28], RBSDV P9-1 self-associates into aggregate formations and preferentially binds ss-RNA molecules [29,30].Given the high homology between SRBSDV P9-1 and RBSDV P9-1 [31], SRBSDV P9-1 could have a similar function, and may also be able to bind small molecules.
In our previous studies, we characterized the binding of dufulin with a dissociation constant of ~3.26 -~8.55 μM to the SRBSDV P9-1 oligomer based on the results of isothermal calorimetry (ITC) and microscale thermophoresis (MST) [32,33].Dufulin, a novel antiviral agent of α-amino phosphonate, inhibits the replication of plant viruses [34].In order to optimize and develop anti-SRBSDV compounds with high affinities of SRBSDV P9-1, our lab synthesized a series of α-amino phosphonate derivatives [35].
Herein we focus our studies on analyzed the crystal structure of SRBSDV P9-1 using X-ray crystallography and investigated the interaction studies of α-amino phosphonate derivatives and SRBSDV P9-1 octamer using Fluorescence Titration (FT), ITC and MST.With the purpose of providing important help for the further improvement of the lead compound of α-amino phosphonate derivatives, P9-1 structure was used as a target.

Expression, purification and crystallization of SRBSDV P9-1
To prepare SRBSDV P9-1 for crystallization, SRBSDV P9-1 genes were overexpressed when the final concentration was increased to 0.7 mM isopropylβ-D-galactopyranoside (IPTG) and the solution was left overnight at 16°C.More than 90% of the P9-1 protein was eluted.Then, the GST-tagged was cut overnight at 4°C by prescission protease, and the dealt proteins were loaded in size exclusion chromatography (SEC) column, in a buffer containing 10 mM Tris-HCl and 100 mM sodium chloride pH 7.5, the retention time of the SRBSDV P9-1 protein was determined with octameric formation (Supplementary Figure 1) [31].The final yield from 500 ml of bacterial culture was about 3.5 mg of purified SRBSDV P9-1 protein after using a desalting column.SRBSDV P9-1 crystals were crystallized using hanging-drop vapor diffusion, most crystals of SRBSDV P9-1 with a typical maximal dimension of 0.2 mm × 0.3 mm within 10 days.

Overall architecture of SRBSDV P9-1
SRBSDV P9-1 crystals belong to the orthorhombic space group C2221, with unit cell dimensions a = 120 Å, b = 152 Å and c = 146 Å (Supplementary Table 1).The structure was solved by molecular replacement and refined to a resolution of 2.2 Å (PDB accession code: 5EFT).The eight subunits of the asymmetric unit were individually refined by simulated annealing, as indicated by the root mean square deviation (RMSD) between the individual subunits (0.01-0.02Å).The secondary structure of each subunit in the refined atomic model was very similar to that described previously by Akita et al [28], with RMSDs of ~ 0.6 Å, ~1.6 Å and ~1.6 Å for the carbon-alpha backbone (not including the loop region).The residue of the monomer densities corresponding to fitted coordinates were supplied in Supplementary Figure 2. The wellordered portions had similar secondary structure in all eight subunits in the octamer [28].

Comparison with the reported RBSDV P9-1 secondary structure
The secondary structure of SRBSDV P9-1 was consistent with the previously reported structure of RBSDV P9-1, part form the presence of helix in 332-325 aa (α9) at the C-terminus and five additional b-sheets in 13-19 aa (β1), 43-49 aa (β2) , 113-117 aa (β3) , 121-127 aa (β4) and 163-168 aa (β5) (Figure 4) at the N-terminus that form a novel β1-5 region.The major differences from the previously reported RBSDV P9-1 structure were the manner of subunit assembly and the specific proteinprotein interactions in the major overlap and internal overlap regions.Superposition of the core of the protein backbone carbon-alpha atoms (RMSD of ~1.1 Å) confirms the similar overall folding octameric structure.The ordered segments of the A-and a-chains can be superimposed on each other by a rigid body translation with a RMSD of 0.2 Å for the carbon-alpha atoms.All helical residue densities corresponded to the fitted coordinates, and the electron density for large polar and charged amino acids and some small polar and charged residues and basic side chains were in clear with the fitted coordinates.A 2Fo-Fc electron density map of the SRBSDV P9-1 octamer contoured at the 1.0 σ level, and the amino acid sequence numbers are included.
Four obvious interactions among subunits were visible in the SRBSDV P9-1 structure, and these were similar to the four inter-subunit interactions observed in the RBSDV P9-1 structure, although there were a greater number of hydrogen bonds in the SRBSDV P9interactions.The interactions between A2 and A3, with five hydrogen bonds in main chains (Figure 3A), the interactions between A2 and A1, with five hydrogen bonds in main chains (Figure 3B), the interactions between A2 and a1, with four hydrogen bonds in side chains (Figure 3C), the interactions between A2 and a2, with six hydrogen bonds in side chains (Figure 3D).In RBSDV P9-1, there were also three interactions between one monomer and another, but there were less hydrogen bonds in this three interactions, the interactions between A2 and A3, with only two same hydrogen bonds in main chains, Asn310 and Ile336; Glu313 and Asn33, the interactions between A2 and a1, with four different hydrogen bonds inside chains, Lys344 and Asn247; Leu343 and Asn247; Lys201 and Ser341; Leu342 and His77, the interactions between A2 and a2, with only three hydrogen bonds (two different, one same) in inside chains, Glu221 and Arg216; Asn258 and Arg216; Asp262 and Asn258 (same).
Six major differences were present in the ß-sheet regions of RBSDV P9-1 and SRBSDV P9-1, suggesting the charged and polar residues (K20R, D109V, T164F, L123V, L124C, T128V) in these positions was involved in binding small molecules or nucleic acids, and was negatively or positively charged as appropriate for forming the corresponding complex.In addition, in the pore of the structure of the SRBSDV P9-1 octamer, the longer ß-sheet structures in the P9-1 cylindrical octamer core were disordered (Figure 2C).This may be due to the fact that the core structure forms five bundles of the charged and polar amino acid residues (KIEELTIK, TKKIPLLD, YDR, TKTEMS and LLLEYTL) (Figure 6).

The affinities of SRBSDV P9-1 octamers and α-amino phosphonate derivatives
To investigate the binding studies of α-amino phosphonate derivatives and the P9-1 proteins, we focus our studies on the interactions between six α-amino phosphonate derivatives and the P9-1 proteins using FT methods, the constants of all six α-amino phosphonate derivatives binding to the P9-1 proteins were listed in Supplementary Table 2, the quenching constants for the interaction obtained by the Stern-Volmer method were listed.The binding constant between six α-amino phosphonate derivatives and the P9-1 proteins can be calculated by employing the Lineweaver-Burk equation lg (F 0 -F) / F = lgK a + n lg[Q].The curves of 1/(F 0 -F) versus 1/[Q] were linear.As a rule, for dynamic quenching, the maximum scatter collision-quenching constant of various quenchers is 2.0×10 10 L•mol -1 •S -1 [36].
Two advanced techniques, ITC and MST, were utilized in the experiments allowing us to prove the interaction results by FT in vitro.The ITC responses recorded during titration of the P9-1 proteins with GUFCC-013 and GUFCC-023 were shown in Figures 7C and 7D.Binding studies for interactions of the P9-1 proteins with GUFCC-013 and GUFCC-023 were listed in Supplementary Table 3. Obviously, the GUFCC-013-P9-1 and GUFCC-023-P9-1 with a K a of 9.12×10 4 and 1.41 × 10 5 exhibited higher binding affinities, showed similar affinities with dufulin [32], it was consistent with the results of fluorescence.The results showed that the GUFCC-013 and GUFCC-023 bound to the P9-1 proteins with a dissociation constants (K d ) of 6.01 (K a = 1.66 × 10 5 ) and 2.88 (K a = 3.47 × 10 5 ) µM, respectively (Figures 7E and 7F), The binding datas determined from MST experiments were listed in Supplementary Table 4. Determined K d obtained in ITC and MST experiments were corresponding to data obtained by means of FT.All the results indicated that α-amino phosphonate derivatives containing 4-chlorophenyl group and phenyl group will provide important help for the further improvement of the lead compound and develop to the potential anti-SRBSDV drugs.
Molecular docking results showed that the P9-1 interacts with GUFCC-023 through Arg237 (Supplementary Figure 9), and then we expressed the mutated P9-1 (R327A) protein by site mutation.MST results showed that the GUFCC-023 bound to the mutated P9-1 (R327A) with a K d of 10.97 µM (n = 1, K d confidence = 2.77), The binding affinities were significant differences between the mutated P9-1 (R327A) and wild type P9-1 (2.88 µM in Supplementary Table 4).Furthermore, the Arg327 was the key residue to form the dimmer of P9-1 protein.The results indicated that the Arg327 was key residue for GUFCC-023 binding.

DISCUSSION
The P9-1 expressed without a tag was capable of forming high-quality crystals suitable for X-ray diffraction studies.When P9-1 was expressed with an N-terminal 6 × His tag or truncated at the C or N terminus, crystals did not form.The reason for these was remained to be determined.It was possible that these proteins formed a heterogeneous mixture of monomers, dimers, tetramers, and octamers that were less amenable to forming a wellordered crystal lattice, except deleted tags.
In the crystal structure of SRBSDV P9-1 determined in the present study, 16 main interactions among subunits form an octamer.A number of polar and charged amino acids lie in the core of SRBSDV P9-1 octamer formed from the ß-sheet regions.The electron density map indicated that some residues in this region were disordered in the SRBSDV P9-1 octamer core.Interestingly, The 2.2 Å structure was a higher resolution than that reported for the homologous RBSDV P9-1, and a greater number of polar and charged residues and interactions were visible in the core of the SRBSDV P9-1 structure compared with RBSDV P9-1.Furthermore, the conformation and overall structure SRBSDV P9-1 appeared to be more complex than that of RBSDV P9-1.
These polar and charged residues in the SRBSDV P9-1 octamer core were likely to adopt an ordered structure when it bound to P9-1 DNA segments (we will report in other article), and keep the activity of the P9-1 octamers.In our previous reported, we found α-amino phosphonate compound-dufulin had a micromole affinity with SRBSDV P9-1, and were corresponding to the activity data in vivo, so α-amino phosphonate compound was a good ligand target for P9-1 octamers.
Then we focus our studies on the crystal structure of the P9-1 as the target for α-amino phosphonate derivatives.Using FT, ITC and MST methods, we screened two α-amino phosphonate derivatives GUFCC-013 (containing 4-chlorophenyl group) and GUFCC-023 (containing phenyl group) which exhibited high binding affinities.This finding implies that it is possible to develop α-amino phosphonate derivatives containing 4-chlorophenyl and In summary, the results presented here reveal that the high resolution crystal structure of the SRBSDV P9-1 octamer was analyzed and applied it as the target of α-amino phosphonate derivatives.In addition, GUFCC-013 and GUFCC-023 was screened with high proteins in the presence of increasing GUFCC-013, the results showed that one P9-1 combined one GUFCC-023 with a dissociation constant (K d ) of 11 nM, and the corresponding Ka was 9.12 × 10 4 M -1 .(B) Fluorescence emission spectra of P9-1 proteins in the presence of increasing GUFCC-023, the results showed that one P9-1 combined one GUFCC-023 with a Kd of 7.09 nM, and the corresponding Ka was 1.41 × 10 5 M -1 .(C) ITC results showed that one P9-1 combined one GUFCC-013 with a Kd of 10.9 nM, and the corresponding Ka was 9.17 × 10 4 M -1 .(D) ITC results showed that one P9-1 combined one GUFCC-023 with a Kd of 4.12 nM, and the corresponding Ka was 2.43 × 10 5 M -1 .(E) MST results showed that one P9-1 combined one GUFCC-023 with a Kd of 6.01 nM.(F) MST results showed that one P9-1 combined one GUFCC-023 with a Kd of 2.88 nM.www.impactjournals.com/oncotargetbinding affinities to P9-1 octamer, and will develop into the potential anti-SRBSDV drugs.

The procedure for recombinant P9-1
Rice plants infected with SRBSDV were gathered from Guizhou province, China.The SRBSDV total RNA was extracted using the trizol reagent method, and then a spectrophotometer was used to detect trace amounts of nucleic acid.The cDNA synthesis methods were described in a previous report [31].
SRBSDV genomic RNAs were isolated according to previous published methods [31] and were reverse transcribed in the presence of a 9 nt random primer with superscript reverse transcriptase (Invitrogen, USA) according to the manufacturer's instructions.PCR amplification was carried out using iCycler (Bio-Rad, USA) under the following conditions: 94°C for 3 min; 20 cycles at 94°C for 50 s, 65°C for 50 s, and 72°C for 50 s, then was followed by 25 cycles at 94°C for 50 s, 55°C for 50 s, and 72°C for 50 s, with a final incubation time of 10 min at 72°C.The reaction was performed in a 50 μL solution containing 400 ng cDNA, 4.0 mM MgCl 2 , 1 mM dNTP, 0.8 μM of each primer and 2.5 U Taq polymerase (TaKaRa, Japan).The complete P9-1 gene sequence (GenBank accession no.AEB40349) for SRBSDV was used to design primers containing BamHI restriction site in the forward primer (CGGGATCCATGGCAGACCTAGAGCGTA) and EcoRI site in the reverse primer (CCCTCGAGTCAAACGTCCAATTTAAGTGAAG).
The full-length P9-1 gene was digested with BamHI and EcoRI and then inserted into the vector pGEX-6P-1 (Novagen, USA) cut with the same enzymes, resulting in the prokaryotic expression plasmid pGEX-6P-1-P9-1.The sequence of P9-1 was confirmed by DNA sequencing in Shanghai Sangon Company, China.Sequence analysis showed that SRBSDV P9-1 contained 1044 nts and shared a 99% nucleotide identity with other published SRBSDV isolate sequences in the National Center of Biotechnology Information (NCBI) (GenBank accession no.KJ476858).
The expression vector, pGEX-6P-1-P9-1, containing the full-length P9-1 gene, was designed and used to transform Escherichia coli strain BL21(DE3)RIL (Novagen, USA).A freshly transformed overnight culture of Escherichia coli strain BL21(DE3)RIL containing the plasmid pGEX-6P-1-P9-1 was transferred to 1 L Luria broth.The cells were grown at 37°C in Luria-Bertani medium supplemented with 100 μg/ml Ampicillin, and with an OD 600 of 0.65.The cells were shaken at 200 rpm.Then protein expression was induced with 0.7 mM IPTG at 16°C overnight.The cells were harvested by centrifugation and then stored at -80°C.When analyzed, the cells were resuspended in lysis buffer (20 mM Tris-HCl, 500 mM NaCl, 30 mM imidazole, 5 mM β-mercaptoethanol and 5% glycerol, pH 7.5) and then lysed at 4°C by sonication.The lysate was clarified by centrifugation at 12, 000 g for 30 min at 4°C, the soluble supernatants were loaded onto a 5 ml Ni-NTA column (GE Healthcare, USA), and the protein was eluted with a linear gradient of 30-350 mM imidazole (pH 7.5).The crude protein was performed at 4 °C using a desalting column (GE Healthcare, USA) attached to an AKTA purifier protein liquid chromatography system (GE Healthcare, USA), and the fractions containing target protein with His-and GST-tags were pooled, concentrated to a suitable concentration by ultrafiltration (10 kDa cut-off), and the GST-tag was digested off by prescission protease at 4°C (1 mg prescission protease with 100 mg GST-tagged fusion protein).The dealt protein concentration was determined using a Genequant100 (GE Healthcare, USA), and stored at -80°C until further analysis.

Crystallization and Structure Determination of SRBSDV P9-1
Crystallization was performed at 20°C by using the hanging-drop vapor diffusion method.SRBSDV P9-1 protein was concentrated to 3.5 mg/mL in a buffer containing 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, and 1 mM DTT, and then crystallization conditions were carefully screened [38].Briefly, 1 µL protein solution was mixed with 1 µL reservoir solution (100 mM Trimethylamine N-oxide dihydrate, 100 mM Tris-HCl, pH 7.5, 16% w/v Polyethylene glycol monomethyl ether 3000) and equilibrated against 0.6 mL reservoir solution.The micro-crystals were grown for 10 days and collected and deposited with a seeding tool (Hampton Research, USA) [38].Many SRBSDV P9-1 crystals have been crystallized using hanging-drop vapor diffusion within 10 days.High-quality crystals of SRBSDV P9-1 were www.impactjournals.com/oncotargetmounted and flash-frozen in liquid nitrogen following cryo-protection with reservoir solution containing an additional 25 % (w/v) Polyethylene glycol monomethyl ether 3000 [38].
Diffraction data were collected at the Shanghai Synchrotron Radiation Facility (SSRF) beamline 17U.All X-ray data were processed using the HKL2000 program [39] and converted to structure factors within the CCP4 program [40].A typical octahedral-shaped crystal belongs to the space group C2221.The structure was solved by molecular replacement in Phaser [41] using the published RBSDV P9-1 monomer as a search model (PDB code 3VJJ) [28].The SRBSDV P9-1 model was manually built in COOT [42], and computational refinement was conducted with the program REFMAC5 [43] in the CCP4 suite.Molecular graphic figures were prepared in PyMOL [44], the eight subunits of the asymmetrical unit were refined individually using simulated annealing.There were no departures from the noncrystallographic symmetry found during this procedure of refinement, as indicated by the RMSD between the individual subunits (0.01-0.02Å) [45].

Interaction studies between NNM and TMV CP
All fluorescence measurements were made on FluoroMax ® -4 and FluoroMax ® -4P (Horiba Scientific, France).The excitation wavelength was 275 nm while the emission spectra were recorded from 280 nm to 400 nm.Both the excitation and emission slit widths were set at 5 nm.The concentration of P9-1 proteins for each run was fixed at 10 μM, the concentration of α-amino phosphonate derivatives, respectively 0, 2, 4, 6, 8, 10, 12, 14, 16 and 18 μM.The decrease in fluorescence intensity at the highest peak was analyzed according to the Stern-Volmer equation [46]: where F 0 and F are the fluorescence intensities in the absence and presence of a quencher (α-amino phosphonate derivatives), respectively, K q is the fluorophore quenching rate constant, Ksv is the Stern-Volmer quenching constant, τ 0 is the lifetime of the fluorophore in the absence of a quencher (τ 0 = 10 −8 ), and [Q] is the concentration of quencher [47].The binding constants and the binding sites for the complex formation between α-amino phosphonate derivatives and P9-1 proteins were obtained from [48]: lg (F 0 − F)/F = lgK a + n lg [Q].Where K a is the binding constant and n is the number of binding sites.
For the ITC analysis, The ITC binding experiments were performed using an ITC 200 Micro Calorimeter (GE Health, USA) at 25°C.The proteins were dialyzed against a buffer for 24 h before preparing the SRBSDV P9-1 protein.The buffer contained 10 mM Tris-HCl and 100 mM sodium chloride (pH 7.5).The α-amino phosphonate derivative ligands were titrated against the SRBSDV P9-1 protein in a 200 µL sample cell using a 40 µL microsyringe as follows: 0.4 µL for the first injection and 2 µL for the next 19 injections at an interval of 150 s.The integrated heat data were analyzed using the one-setof-sites model in MicroCal Origin 7.0, according to the manufacturer's instructions.The first data point was not used in the analysis.The binding parameters, ΔH (reaction enthalpy change in cal/mol), K (binding constant in mol -1 ) and n (number of molecules per SRBSDV P9-1 or mutant) were floated during the fit.The binding free energy (ΔG) and the reaction entropy (ΔS) were calculated using the equations, ΔG = -RTlnK (R = 1.9872 cal/mol/K, T = 298 K) and ΔG = ΔH -TΔS.The dissociation constant Kd was calculated as 1 / K [49].
For the MST analysis [50], the binding was calculated for MST Monolith NT. 115 (Nano Temper Technologies, Germany).A range of ligands from 0 µM to 5 µM was incubated with 0.5 µM of purified recombinant proteins for 5 min with a NT-647 dye (Nano Temper Technologies, Germany) and was used in the thermophoresis experiment at a final concentration of 20 nM.A 16 point dilution series was made for selected compounds in DMSO.Each compound dilution series was subsequently transferred to protein solutions in 10 mM Tris-HCl and 100 mM sodium chloride pH 7.5, 0.05% Tween-20.After a 15 min incubation of the labeled P9-1 with each dilution point (1:1 mix) at room temperature, samples were filled into standard capillaries (NanoTemper Technologies, Germany).Measurements were taken on a Monolith NT.115 microscale thermophoresis system (NanoTemper Technologies, Germany) under a setting of 20% LED and 40% IR laser.Laser on time was set at 30 s, and laser-off time was set at 5 s.The Kd values were calculated from the duplicate reads of three separate experiments using the mass action equation in the Nano Temper software.
For the docking experiments analysis, the initial structure of SRBSDV P9-1 was revised by adding lost residues and hydrogen atoms, and by checking bonds and bumps.Subsequently, energy was minimized in the steepest descent calculations and conjugated gradient calculations were undertaken using Discovery studio 4.5 (Accelrys, CA, USA).The optimized geometries were used to construct the whole structures.The final structures were optimized by fixing the macrocycle with a conjugated gradient.Atomic Gasteiger-Huckel charges were assigned to the ligand and receptor.Most of the parameters for the docking calculation were set to the default values recommended by the Discovery studio 4.5 software.Each docked structure was scored by the built-in scoring function and was clustered by 0.8 Å of RMSD criteria.The docking analysis results were used to determine the electrostatic potential and partial atomic charges by performing electrostatic potential fitting, according to the Merz-Singh-Kollman scheme with the Gaussian-optimized geometries [51].www.impactjournals.com/oncotargetdwarf virus, a plant reovirus, can be achieved in rice plants by RNA silencing against the gene for the viroplasm component protein.Virus Res.2011; 160:400-403.

Figure 1 :
Figure 1: Crystal structure of SRBSDV P9-1 octamer.(A) Electrostatic surface presentation of SRBSDV P9-1 cylinder octamer of ~83.33 Å in height and ~95.14 Å in width.Positively and negatively charged regions were colored blue and red, respectively.The electrostatic surface was calculated in pyMOL.(B) Electrostatic surface presentation of SRBSDV P9-1 octamer of ~119.51Å in diameter.(C) Own of two P9-1 monomers form dimers via hydrophobic interactions; four P9-1 neighboring dimers form a hollow octamer via carboxy-terminal regions, there was a diameter axial pore with ~39.51 Å at bottom panel.(D) The P9-1 hollow octamer existed a diameter axial pore with ~18.48 Å at top panel, and D was rotated for 180 degrees of C.

Figure 3 :
Figure 3: The interactions involved in subunits of SRBSDV P9-1 octamer.(A) The interactions between A2 and A3, with five hydrogen bonds in main chains in a partial enlarged details, Asn310 and Ile336 (one bonds); Asn310 and Val335 (one bonds); Arg281 and Ser334 (one bonds); Glu313 and Arg216 (two bonds).(B) The interactions between A2 and A1, with five hydrogen bonds in main chains with a partial enlarged details, Asn310 and Ile336 (one bonds); Asn310 and Val335 (one bonds); Arg281 and Ser334 (one bonds); Glu313 and Arg216 (two bonds).(C) The interactions between A2 and a1, with four hydrogen bonds in side chains with a partial enlarged details, Asp346 and Asn247 (two bonds); Arg322 and Ser41 (one bonds); Glu16 and Arg325 (one bonds).(D) The interactions between A2 and a2, with six hydrogen bonds in side chains with a partial enlarged details, Gln252 and Gln267 (two bonds); Tyr259 and Gln267 (two bonds); Asp257 and Arg216 (one bonds); Asp262 and Asn258 (one bonds).

Figure 4 :
Figure 4: Alignment of the amino acid sequences of SRBSDV P9-1 and RBSDV P9-1.Secondary structural elements are indicated schematically under the sequences, generated by EMBL-EBI sequence alignment programs server, and indicated as difference residues (blue box) or spirals (α helices) or arrows (β sheets).