Suppression of breast cancer metastasis through the inactivation of ADP-ribosylation factor 1

Metastasis is the major cause of cancer-related death in breast cancer patients, which is controlled by specific sets of genes. Targeting these genes may provide a means to delay cancer progression and allow local treatment to be more effective. We report for the first time that ADP-ribosylation factor 1 (ARF1) is the most amplified gene in ARF gene family in breast cancer, and high-level amplification of ARF1 is associated with increased mRNA expression and poor outcomes of patients with breast cancer. Knockdown of ARF1 leads to significant suppression of migration and invasion in breast cancer cells. Using the orthotopic xenograft model in NSG mice, we demonstrate that loss of ARF1 expression in breast cancer cells inhibits pulmonary metastasis. The zebrafish-metastasis model confirms that the ARF1 gene depletion suppresses breast cancer cells to metastatic disseminate throughout fish body, indicating that ARF1 is a very compelling target to limit metastasis. ARF1 function largely dependents on its activation and LM11, a cell-active inhibitor that specifically inhibits ARF1 activation through targeting the ARF1-GDP/ARNO complex at the Golgi, significantly impairs metastatic capability of breast cancer cell in zebrafish. These findings underline the importance of ARF1 in promoting metastasis and suggest that LM11 that inhibits ARF1 activation may represent a potential therapeutic approach to prevent or treat breast cancer metastasis.

In prostate cancer, we have linked ARF1 to the hyperactivation of mitogen activated protein kinase (MAPK) Raf1/MEK/ERK1/2 pathway [7]. In breast cancer, ARF1 has been reported to be involved in promoting cell proliferation and migration via multiple well-known signaling cascades. For example, ARF1 controls cell adhesion by regulating the recruitment of key focal adhesion proteins (such as paxillin, talin and FAK) to β1-integrin [17], controls cell proliferation by regulating pRB/E2F1 activity and gene expression [15], and controls cell migration and invasion by regulating the activation of Rac1 and PI3K/AKT pathways [14,16]. Most recently, ARF1 is shown to act as a molecular switch to activate EGF-mediated responses and mediate the sensitivity of triple negative breast cancer cells to EGFR tyrosine kinase inhibitors [18]. On the basis of these clues, we wondered whether ARF1 functions as a metastasis promoter in breast cancer to drive metastasis. Here we provide evidence for such a role, and demonstrate that ARF1 is a very compelling target to limit breast cancer metastasis. Inactivating ARF1 may have a potential therapeutic value in this regard.

High-level amplification of ARF1 gene in breast cancer
To explore whether the ARF genes contribute to the occurrence and development of cancer, we investigated the genetic alteration of a panel of the ARF gene family using publicly accessible TCGA datasets (see Materials and methods). Intriguingly, amplification of the ARF genes was found in 17% of cases of breast cancer, which was the highest in all the examined cancer types ( Figure 1A). Amplification was the predominant type of alteration for ARF1 gene and its frequency was much higher (14% of cases) than other family members in breast cancer. Functional plotting of the corresponding mRNA level in relation to genetic status of ARF1 revealed that amplification of ARF1 was associated with increased mRNA expression ( Figure 1B). To further validate these findings, the relative expression of the ARF1 transcript was examined in breast cancer entities from the Oncomine database, which showed ARF1 expression levels were significantly higher in cancer than normal tissues ( Figure 1C). Univariate survival analysis (Kaplan-Meier method and log-rank test) revealed that breast cancer patients with low levels of ARF1 expression significantly improved relapse-free survival as compared with high expression levels ( Figure 1D), which is likely to be related to its involvement in the lethal and advanced forms of breast cancer.

ARF1 is upregulated in human breast cancer tissues
To validate the ARF1 expression pattern at protein levels, breast cancer tissue microarrays were used for immunohistochemistry (IHC) analysis. Our data indicate remarkably increased levels of ARF1 in primary breast cancer tissues compared with normal breast epithelium, and strong membrane staining of ARF1 in advanced breast cancer (Figure 2A). Most interestingly, higher levels of ARF1 were associated with higher cancer stages ( Figure 2), supporting its critical role in breast cancer progression.

Loss of ARF1 expression suppresses metastasis in breast cancer
To better understand the role of ARF1 in breast cancer, we used shRNA constructs to inhibit ARF1 expression in high-invasive breast cancer MDA-MB-231 cells ( Figure 3A). Using two different shRNA constructs, knockdown of ARF1 led to significantly reduced potential in cell invasion within 24 hours ( Figure 3B) with modest decreased cell proliferation. To explore the importance of ARF1 in metastasis in vivo, MDA-MB-231 cells with ARF1 knockdown were injected into the mammary fat pad of NSG mice and metastasis was monitored in these orthotopic breast cancer models. When pulmonary metastasis was examined at the conclusion of the experiment, mice injected with the knockdown control cells showed more nodules on the lung surface with heavier weights ( Figure 3C and 3D), compared with those injected with ARF1 knockdown cells. Histological analysis of these lungs further revealed a notable decrease in the number and size of metastatic foci on lung section when ARF1 was depleted ( Figure 3E).
Owing to the transparent and immunoprivileged nature of zebrafish embryos, we have recently established a zebrafish-metastasis model through a critical evaluation of various types of human cancer cells [19][20][21]. To determine whether the phenotype results from ARF1 knockdown is reproducible in the zebrafish-metastasis model, we generated tumor-bearing zebrafish through injecting ARF1 knockdown MDA-MB-231 cells and the knockdown control cells. Consistent with the findings from NSG mice, metastatic dissemination of the knockdown control cells was seen in 76% zebrafish at 2 days post-injection (dpi), whereas ARF1 knockdown cells were only disseminated in 24% of zebrafish ( Figure 3F). These observations demonstrate that ARF1 is required for breast cancer progression and acts as a metastasis promoter.

LM11 inhibits ARF1 activation in breast cancer cells in a dose-dependent manner
We next determined levels of ARF1 expression and activation status in well-established human breast cancer cell lines. Real-time RT-PCR revealed elevated expression levels of ARF1 in cancer cells (MCF7, MDA-MB-231 and Hs578T) when compared to human MCF10A mammary epithelial cells ( Figure 4A). Moreover, the expression levels of ARF1 in two triple-negative cell lines (MDA-MB-231 and Hs578T) were much higher than those in MCF7 cells ( Figure 4A). Interestingly, the levels of active GTP-bound ARF1 were strongly correlated with ARF1 expression in breast cancer cells ( Figure 4B).
The data shown above suggest that inhibiting ARF1 expression may be a means of suppressing aggressive stage of breast cancer. However, there are no drugs directly targeting ARF1 expression. ARF1 is a small GTPase and its function largely dependents on the active form [7,9,10], therefore, we investigated the efficacy of the inhibitors blocking ARF1 activation. We have previously reported that a small molecule LM11 can effectively impair ARF1 activation in HeLa cells through targeting   ARF1-GDP and ARF nucleotide-binding site opener (ARNO) in regions close to the ARF1/ARNO interface [22,23]. As shown in Figure 4C, LM11 also inhibited ARF1 activation in breast cancer cells, and this effect was dose dependent.

LM11 inhibits cell viability and invasion in breast cancer cells
To specifically evaluate the effect of LM11 on breast cancer, we determined the in vitro cytotoxicity of LM11 in three breast cancer cell lines. Cell viability assays using CellTiter-Glo ® Luminescent cell viability kit (Promega, Madison, MI) showed that the IC 50 (50% inhibitory concentration) of LM11 in the examined breast cancer cell lines ranged from 40 µM (MDA-MB-231 and Hs578T) to 75 µM (MCF7) ( Figure 5A). Flow cytometry-based analysis further indicated, unlike high dose (50 µM), low dose of LM11 (10 µM) only modestly affected cell viability of breast cancer cells ( Figure 5B). We thus determined LM11 effect on cell motility at low dose. Gap closure migration assays showed that LM11 significantly inhibited cell migration in both MDA-MB-231 and Hs578T cells ( Figure 5C and 5D). Moreover, transwell invasion assays demonstrated that LM11 treatment led to a sharp decreased in invasion potential in these cells ( Figure 5C and 5E). These data indicate that LM11 exhibits potent in vitro cytotoxicity and suppression of invasion in breast cancer cells.

LM11 effectively suppresses breast cancer metastasis
The zebrafish-metastasis model can evaluate the metastatic ability of cancer cells [19][20][21]24]. We have shown above (Figure 3) that zebrafish robustly reported the decreased metastasis potential in breast cancer cells where the ARF1 gene was knocked down. Therefore, we used this model to evaluate the efficacy of LM11 in suppression of breast cancer metastasis. To ensure that the effect was specific to tumors and did not affect the zebrafish development and growth, the overall length of each fish was recorded under bright field illumination before and after LM11 treatment. At the concentration ranging from 0.1-1 µM, LM11 did not alter overall fish growth within 1 week of treatment, as compared with the treatment of vehicle (DMSO) (data not shown). Most importantly, 1 µM of LM11 significantly inhibited MDA-MB-231 cells to disseminate from the perivitelline cavity to fish body ( Figure 6). These observations demonstrate that LM11 has strong anti-cancer activities through suppressing the phenotypes associated with breast cancer metastasis.

dIscussIon
ARFs are a family of GTPases involved in a range of cellular functions, including critical functions in vesicular transport [25]. Here we show that ARF1 is often abnormally overexpressed in breast cancer cells, and such overexpression is crucial to promote invasion and metastasis to be significantly associated with the poor outcomes of patients. Based on these facts, we demonstrate that ARF1 serves as a previously unidentified drug target and inactivating it can suppress metastatic breast cancer. The study of breast cancer metastasis is always hampered by a lack of reliable metastatic models. Although the importance of the ARF1 gene has been determined using tail vein injection of immunocompromised SCID mice [26], intravascular injections only model extravasation and metastasis, and lack the growth characteristics and metastatic properties of human cancer [27]. Considering that NSG mice allow primary and metastatic tumors to develop coincidently [28], we established orthotopic breast cancer models in NSG mice to illustrate the contribution of ARF1 alteration to cancer progression. Orthotopic implantation in mice indicates that loss of ARF1 expression in breast cancer cells reduces risk of metastatic spread. These findings, together with the investigation in zebrafish, provide more solid evidence that ARF1 promotes breast cancer metastasis.
Mutations in RAS genes are very rare in human breast cancers, but RAS is pathologically hyperactivated in half of these cancers [29][30][31]. Since approaches to directly target RAS have not been successful, most efforts to block activated RAS have focused on pathways downstream, such as the MAPK and PI3K pathway [32,33]. However, targeting these nodes in the signaling cascades individually typically involves a switch to the other pathway in a rescue strategy by the cancer cells to overcome monotherapies [34][35][36][37]. Previous studies demonstrate, similar to the oncogene RAS, ARF1 directly and activation-dependently activates the MAPK and PI3K pathway [7,14,26]. Therefore, inhibition of ARF1 may provide an alternative over the generally toxic simultaneous inhibition of multiple pathways by different drugs.
The exchange of GDP for GTP on ARFs is catalyzed by guanine nucleotide exchange factors (GEFs), and ARF-GEFs interact with ARFs through their Sec7 domain, which is necessary and sufficient for GEF activity [38][39][40]. Most existing ARF1 inhibitors block its activation by targeting the Sec7 domain on ARF1-GEFs [22]. Although inactivating a subset of ARF1-GEFs has been very useful for assessing the ARF1 function, ARF1 inhibitors remain a daunting challenge to develop into anti-cancer and anti-inflammatory drugs. For example, Brefeldin A (BFA), a known inhibitor stabilizing an abortive complex between the GEFs and ARF1 in the GDP-bound form, is highly cytotoxic [40]. Unlike BFA, Exo2 interferes with the function of ARF1 or ARF1-GEFs localized to the ER-Golgi intermediate compartment or the trans-Golgi network. While compared to BFA, Exo2 reduces ARF1 activation but the effect is much weaker [41]. Recently, a novel ARF1-ARFGEF inhibitor AMF-26, which differed structurally from BFA, has shown potential for inducing complete regression of human breast cancer BSY-1 xenografts in vivo [42]. However, no evidence has been provided that AMF26 and its derivatives has anti-metastasis effects. We have characterized a compound, LM11, specifically targets the ARF1-GDP/cytohesin-2 complex and acts as a non-competitive inhibitor [22]. In MDCK cells, LM11 suppresses ARNO-dependent migration because of its inhibition of ARF1 functions at the Golgi [22]. The data present here, for the first time, show potential therapeutic opportunities for breast cancer resulting from ARF1 inactivation in the context of LM11 treatment.
Recently, more general cancer characteristics such as genomic instability, invasiveness, and transplant ability apply to zebrafish tumors [19-21, 24, 43, 44]. As a model of whole organism, zebrafish can be used to facilitate better understanding of gene function involved in cancer progression and provide a means to develop promising preclinical agents. We evaluated ARF1 function and LM11 efficacy using the zebrafish-metastasis model. The value of fish assays is that the results can be obtained in an informative, cost-effective and time-efficient manner. The mice, therefore, will be used to confirm the effect of LM11 on suppression of metastasis.

Cell lines and standard assays
Human breast cancer cell lines (MDA-MB-231, Hs578T and MCF7) and normal mammary epithelial cells MCF10A were directly obtained from American Type Culture Collection (ATCC, Rockville, MD). All the cell lines used in this study have been verified using SNP-CGH for characteristic cytogenetic changes or confirmed using STR DNA fingerprinting analysis [45,46]. Standard cell culture, transient transfections, lentiviral transduction, western blot, real-time RT-PCR and cell proliferation assays were carried out as described previously [28,[45][46][47].

Cell viability and flow cytometric analysis
The cell viability was determined by CellTiter-Glo ® Luminescent cell viability assay (Promega, Madison, MI) and Zombie Aqua TM fixable viability kit (BioLegend, San Diego, CA) according to manufacturer's instructions. Flow cytometry (LSR Fortessa cell analyzer, BD Biosciences, San Jose, CA) was used to record and analyze the cells stained with Zombie Aqua TM dye with a maximum emission of 516 nm.

Gap closure migration assays and invasion assays
Cell migration was performed using the Radius™ 24well from Cell Biolabs (San Diego, CA). Briefly, cells were seeded on Radius cell migration plates and allowed to form monolayers. Circular gaps were generated by removing the gels and cells were treated with DMSO or LM11 for 24 hours. To compare differences in the migratory gap, phase-contrast images were captured at the same size using a Zeiss LSM-510 inverted microscope (Zeiss, Germany) and gap closure was analyzed using ImageJ. Cell migration velocity was calculated and statistically analyzed from three independent experiments. Cell invasion was performed using a Matrigel-coated modified Boyden chamber (BD biosciences, San Jose, CA) as described previously [45][46][47]. After incubation for 24 hours, cells on the upside were removed using cotton swabs, and the invading cells on the lower side were fixed and stained with 0.2% crystal violet. Numbers of the invading cells in six randomly selected fields were counted in each experiment using a Zeiss LSM-510 inverted microscope.

Experimental metastasis assays
All experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at Augusta University. Tg(kdrl:EGFP) transgenic zebrafish which highlights the vasculature were maintained using established temperature and light cycle conditions as previously described [19,48,49]. In zebrafish metastasis assays, cancer cell transplantation was performed essentially as described previously [19]. Briefly, MDA-MB-231 cells infected with the lentivirus expressing control or ARF1 shRNA were labeled with fluorescent dye CM-Dil (Life Technologies, Carlsbad, CA). Approximately 200 labeled MDA-MB-231 cells expressing ARF1 shRNA or control shRNA were microinjected into the perivitelline space of 2 days post fertilization (dpf) Tg(kdrl:EGFP) embryos. The embryos were kept at 34°C and then imaged under anesthesia by confocal microscopy at 2 dpi. Percentage of metastasis was set as the number of embryos containing more than 5 cells outside the yolk sac. Total metastasis percentage was set as the total number of embryos with metastasis at 2 dpi relative to day zero. In mouse metastasis assays, ARF1 knockdown MDA-MB-231 cells and the control cells were individually injected into 6-weekold female NSG mice (NOD.Cg-Prkdc scid Il2rg tm1Wjl / SzJ, Jackson Laboratory, Bar Harbor, ME) through the mammary fat pad under the fourth (abdominal) nipple as described previously [28]. Mice were sacrificed 6 weeks after injection and the lungs were then fixed in 10% neutral buffered formalin, embedded in paraffin blocks, sectioned at 5 μm, and subjected hematoxylin and eosin (H&E) staining.

Zebrafish drug treatment
Tg(kdrl:EGFP) embryos at 2 dpf were injected with MDA-MB-231 cells and housed in 24-well plates containing a single larva per well in 500 µl filter-sterilized fish water. Fish were administrated with 0.5% DMSO or different doses of LM11 in the fish water at 4 hours postinjection (hpi). After 4 days of treatment, metastasis in fish body was analyzed using confocal microscopy. Percentage of metastasis was set as the number of embryos containing more than 5 cells outside the yolk sac. Total metastasis percentage was set as the total number of embryos with metastasis at 4 dpi relative to day zero. www.impactjournals.com/oncotarget

Tissue microarrays and IHC
The human breast tissue microarrays were purchased from US Biomax (Rockville, MD) and Novus Bio (Littleton, CO). IHC of the human tissue microarrays was conducted as described previously [7] using an ARF1 antibody (1:500). For quantifying staining intensity, 12 random microscopic fields were captured by a CCD camera (Olympus, Center Valley, PA) and signal intensity was determined using the Image-Pro Plus software (MediaCybernetics, Rockville, MD).

Bioinformatics and statistical analysis
To determine the influence of ARF1 expression on relapse-free survival of breast cancer patients, integrated available genome-level transcriptomic datasets from the Kaplan Meier (KM)-plotter [50] were assessed by stratifying patients based on the higher or lower ARF1 expression. The genetic status of human ARF gene family related to cancer types was calculated from TCGA data by cBioPortal (http://www.cbioportal.org/) up to April 2016. The selected datasets in this study must contain a large number of patient samples (> 100). Expression data of ARF1 in normal breast and breast cancer samples were obtained from Oncomine website (www.oncomine.org). Experiments shown are the means of multiple individual points from multiple experiments (± S.D.). A 2-tailed P-value of less than 0.05 was considered to indicate statistical significance.